Problem running fs_etomo_fiducials: wrpDose column not detected

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Moritz Kirchner

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May 12, 2026, 5:02:12 AM (3 days ago) May 12
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Hello,

I am currently processing a tomography dataset and have been having problems with running the fs_etomo_fiducials protocol.
After running fs_motion_and_ctf I have created tomostar files for the project. Then I wanted to align running fs_etomo_fiducials using the following command:

WarpTools ts_etomo_fiducials --settings warp_nanobdella_sha05g6.settings --angpix 10 --fiducial_size 10 --initial_axis 84.8 --device_list 1

However, the protocol fails to process the files and the error logs (attached) show that either the wrpDose and/or wrpTilt column seems to be missing from the tomostar files. When I have a look the the corresponding tomostar file (attached) I can clearly see that the wrpDose column is present and the file has the same columns in the same order as the example given in the Quick start Guide. I am not sure why WarpTools is not detecting the column it should (which I guess is wrpDose and not wrpTilt at this point because wrpTilt is not in the example). Has anyone else had problems with this and would know of a solutions? Many thanks in advance!

Best wishes,
Moritz

sha05g6_squares_10_A1_ts_001.tomostar
sha05g6_squares_10_A1_ts_001_-0.0_Mar20_04.12.31.log
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