Combine dataset

[Chen Sun]

Hello,

I have two small datasets that I processed with warp individually to tomogram alignment. How can I combine these two datasets for the following processes? Do I just copy the content in the warp_tiltseries into one folder? 

Best wishes,

Chen

[Reika Watanabe]

Hi, Chen,

I am not sure that simple copy-and-paste will work.

Do the two datasets have the same pixel size? Do you plan to use these datasets for subtomogram averaging in Relion5 or others?

If they are the same pixel and camera dimensions, the simplest approach is to restart from preprocessing. That is what I usually do for all my datasets, so I do not have to think about it later. If you manually use Etomo for alignment, you can reuse that alignment.

If they have different pixel or camera dimensions (which is not the case for you), you may need to preprocess separately and combine the particles after particle extraction before subtomogram averaging.

I hope this helps!

Reika 

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[Chen Sun]

Hi Reika,

Thank you for your reply. My two datasets were collected with the exact same pixel size. I guess it would be easier to merge the two dataset after picking the particles.

Best wishes,

Chen