Hi community,
I'm trying to use Noise2Map to denoise my tomograms, I followed the tutorial to use command:
WarpTools ts_reconstruct --settings warp_tiltseries.settings --angpix 3.92 --halfamp_tilts
to generate even and odd tomograms, and I used the following command:
Noise2Map --observation1 warp_tiltseries/reconstruction/even --observation2 warp_tiltseries/reconstruction/odd --observation_combined warp_tiltseries/reconstruction --dont_flatten_spectrum --dont_augment --iterations 10000
to perform denoise, then I got this error:
Loading mask... done.
Preparing data:
Set pixel size to 3.92 based on map header.
Preparing 01122021_BrnoKrios_arctis_lam1_pos5_3.92Apx... Unhandled exception. Accord.DimensionMismatchException: Value does not fall within the expected range.
at Warp.Image.Add(Image summands, UInt32 elements, UInt32 batch) in /work/jpma/chenxr/soft/warp_build/WarpLib/Image.cs:line 3205
at Warp.Image.Add(Image summands) in /work/jpma/chenxr/soft/warp_build/WarpLib/Image.cs:line 3234
at Noise2Map.Noise2Map.Main(String[] args) in /work/jpma/chenxr/soft/warp_build/Noise2Map/Noise2Map.cs:line 298
Aborted
I checked my tomograms, .mrc files, with imod header, It shows:
RO image file on unit 1 : ./even/01122021_BrnoKrios_arctis_lam1_pos5_3.92Apx.mrc Size= 16777217 K
Number of columns, rows, sections ..... 2048 2048 1024
Map mode .............................. 2 (32-bit real)
Start cols, rows, sects, grid x,y,z ... 0 0 0 2048 2048 1024
Pixel spacing (Angstroms).............. 3.920 3.920 3.920
Cell angles ........................... 90.000 90.000 90.000
Fast, medium, slow axes ............... X Y Z
Origin on x,y,z ....................... 0.000 0.000 0.000
Minimum density ....................... -0.57512E-01
Maximum density ....................... 0.98546E-01
Mean density .......................... 0.0000
tilt angles (original,current) ........ 0.0 0.0 0.0 0.0 0.0 0.0
Space group,# extra bytes,idtype,lens . 0 0 0 0
0 Titles :
It shows that density is within -1 to 1, and I also checked with python file mrcfile, the max is 0.12836295, and min is -0.09485604. I dont understand why the value from imod and mrcfile are different, but it seems they are all between -1 and 1. Can someone help me out? Thanks!