Fecal coliform (FC) reduction from other primary vermifilters used in this type of application has been documented in the range of 1-3Log10 as shown in the table below, with the 10 field trials in India reporting 1.7x103 ± 6.02x102 cfu/100mL, or less than 1 Log10 away from the greywater requirement of 200 cfu/100mL required in DEQ Chapter 25 section 17. Since the pathogen reduction in a vermifilter is due to the oxidation and decomposition process of microorganisms and earthworms, temperature can have an effect[i]. An Indian study showed that the trend of FC removal ranged from 2.5 to 3.7 Log10 over a temperature range of 15-35C (59-95°F), reaching a maximum of 1.4x103 MPN/100mL over winter with medium strength synthetic wastewater[ii]. Since greywater is typically warm, processing it in the same system as the blackwater in this design will help keep the temperature up in our cold winters. Given the above, it is anticipated that a primary vermifilter followed by 2 secondary recirculating vermifilters will be sufficient to reliably achieve 200 cfu/100mL. If needed, additional stages will be added.

[i] Arora, S., Rajpal, A., Bhargava, R., Pruthi, V., Bhatia, A., Kazmi, A.A., 2014b. Antibacterial and enzymatic activity of
microbial community during wastewater treatment by pilot scale vermifiltration system. Bioresour. Technol. 166, 132-141.
[ii] Arora, Sudipti & Kazmi, A.A.. (2015). The effect of seasonal temperature on pathogen removal efficacy of vermifilter for wastewater treatment. Water Research. 74C. 10.1016/j.watres.2015.02.001.
[iii] Furlong, Claire & Templeton, M. & Gibson, W.. (2014). Processing of human faeces by wet vermifiltration for improved on-site sanitation. Journal of Water, Sanitation and Hygiene for Development. 4. 231. 10.2166/washdev.2014.107.
[iv] Furlong, Claire & Patankar, Rohit & Thakar, Gouri. (2015). The development of an onsite sanitation system based on vermifiltration: the ‘tiger toilet’. Journal of Water, Sanitation and Hygiene for Development. 10.2166/washdev.2015.167.
[v] Furlong, Claire & Patankar, Rohit & Thakar, Gouri. (2015). The development of an onsite sanitation system based on vermifiltration: the ‘tiger toilet’. Journal of Water, Sanitation and Hygiene for Development. 10.2166/washdev.2015.167.
[vi] Furlong, Claire & Patankar, Rohit & Thakar, Gouri. (2015). The development of an onsite sanitation system based on vermifiltration: the ‘tiger toilet’. Journal of Water, Sanitation and Hygiene for Development. 10.2166/washdev.2015.167.