No individual genotype information exist in the input VCF file

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VIJAY LAKHUJANI

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Jan 16, 2020, 3:43:05 AM1/16/20
to verifyBamID

I ran this command 

VerifyBamID --RefVCF my_file.recode.vcf --Reference genome.fa




I encountered this error:

FATAL ERROR -
No individual genotype information exist in the input VCF file my_file.recode.vcf
Exiting due to ERROR:
 
Exception was thrown


Can you please help; I don't understand what is wrong with my VCF file

VIJAY LAKHUJANI

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Jan 16, 2020, 4:33:04 AM1/16/20
to verifyBamID
My VCF looks like this after the header section ( header is present)

#CHROM  POS     ID      REF     ALT     QUAL    FILTER  INFO
1       865694  SNP1-855557     C       T       .       PASS    CR=99.95174;GentrainScore=0.7626;HW=1.01876395E-7
1       871215  SNP1-861078     C       G       .       PASS    CR=99.613525;GentrainScore=0.8838;HW=1.4922899E-6
1       874665  SNP1-864528     G       A       .       PASS    CR=99.95293;GentrainScore=0.8565;HW=0.025534922
1       879317  SNP1-869180     C       T       .       PASS    CR=99.706245;GentrainScore=0.8049;HW=2.4887462E-4
1       881627  SNP1-871490     G       A       .       PASS    CR=99.81791;GentrainScore=0.8320;HW=3.0533134E-29
1       881918  SNP1-871781     G       A       .       PASS    CR=99.47978;GentrainScore=0.8322;HW=0.016212488
1       887801  SNP1-877664     A       G       .       PASS    CR=100.0;GentrainScore=0.8100;HW=0.9854738
1       888659  rs3748597       T       C       .       PASS    CR=99.91327;GentrainScore=0.8461;HW=1.0

Do you think , there is an issue ? Missing columns or INFO flags?

Hyun Min Kang

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Jan 16, 2020, 7:33:41 AM1/16/20
to verifyBamID
Please use the latest version in GitHub. See https://genome.sph.umich.edu/wiki/VerifyBamID

Your VCF file needs to have allele frequency information.

-----------------------------------------------------
Hyun Min Kang, Ph.D.
Associate Professor of Biostatistics
University of Michigan, Ann Arbor
Email : hmk...@umich.edu


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