Possible questions for interview

[E Jervis]

Each group will meet with me for ~ 20 min (at a date/time) to be scheduled
by email.

Possible questions for the Interview include ...

1) What is the expected turnaround time for production runs with each
host

2) What is the final required protein purity

3) In what form should the final product protein be supplied to the
customer

4) What type of reactors should we specify for each host (e.g. CSTR,
airlift, hollow fiber ...)

5) etc ....

I would like groups to try to come up with some interesting (and useful)
questions! Remember you are trying to establish the FUNCTIONAL
SPECIFICATIONS for your preliminary plant design. You may wish to read
ahead in Bailey and Ollis to get some idea of the requirements for a biotech
pilot plant.

Eric

[E Jervis]

Other things to think about

1) in general, researchers only publish their "best" results. This
means that reasonable estimates of true productivity / yields should be
scaled by some factor (to come up with a truly useful and general estimate)

2) your design will go from the "seed bank" stage to final crude protein
purification

3) don't design a "protein specific" stage into your purification
stream; keep it general

4) know what inclusion bodies are in E. Coli production

5) size all process steps with "reality" in mind

... more to follow ....

P.S. any questions or comments to this discussion stream should be posted as
replies to these comments

E Jervis wrote in message ...

[E Jervis]

There seems to be some confusion as to the length of the paper I am
expecting

20 - 25 pages including all tables figures and sketches

You should find at least 3 of 4 papers per host to get an "estimate" of the
yields and kinetics for each host