Yeast Cell Fermentors

[Conrad Gierer]

I've been playing around with the Aspen bioprocess simulations and came across
a number of problems. I'm using the SFERM model as suggested by Kenton which
seems to .

I'm having lots of problems with choosing parameter values for the unit
operations in Aspen.

Here's a quick summary of some of my problems:

1. RE: Cell Disruption (for Yeast Cells) - Model: DISRUPTOR

I tried using the DISRUPTOR model in Aspen since I assumed it would
act as a bead mill. The model requires a number of parameters which
I'm not sure where to find:

These include: Pressure, Model Type (Hetherington?), rate constants
for model, Yield coefficients on released components....

Also, in my simulation, I've found that the DISRUPTOR model destroys
all my yeast and then when I send the stream to the centrifuge there
is no yeast left to separate from the protein (since it has all been
destroyed) and then I get errors for zero yeast flow into the
disruptor.

2. RE: Centrifuge - Model: CENTRI

I'm not sure what to specify for correlation parameters (ie. Coef1 and
Coef2).

Also, does the protein come out in the concentrate stream or in the
effluent.

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any help would be appreciated. THANKS.

Conrad