Fundamentals Of Biophysics Pdf
Eliz Mettert
Noise in measurements, experimental uncertainties, basics of probability distributions, propagation of uncertainties.
Transmission, reflection and epifluorescence microscopy.
Magnification and resolution; contrast techniques; spherical and chromatic aberrations; hints on optical filters and dichroics.
Confocal microscopy: set-up, point spread function, hints on deconvolution, comparison with TIRF and 2-photon microscopy.
Light-matter interaction: fundamentals (also quantum mechanics) and setups for absorption, fluorescence, Raman, and multiphoton excitation. Jablonski diagrams and properties of fluorescence. Organic dyes: chemical structures and exploitation in fluorescence microscopy.
Hints on fluorescent quantum dots and fluorescent proteins (GFP family).
Diffusion and Brownian motion. Techniques in fluorescence microscopy: colocalization, FRAP-like techniques, FRET, FLIM (fundamentals, instruments, phasors), FCS, super-resolution (RESOLFT, STED, F-PALM, SIM), single molecule spectroscopy and tracking.
Prokaryotes vs eukaryotes. General organization of the eukaryotic cell. Cytoplasm: membrane structure and transport, intracellular compartments, cytoskeleton, cell signalling. The nucleus: chromosomal DNA and its organization, the Nuclear Pore Complex and nucleus-cytoplasmic transport. Cell cycle and cell division. Cell death. Biology laboratory techniques (Methods for cells growth and analysis; purification and analysis of proteins; DNA and RNA: methods for transfection; analysis of genes expression and function; labelling of molecules of interest: strategies and limits)
Providing (also theoretical) bases for quantitatively grasping experiments in molecular and cellular Biophysics at the nanoscale; giving a common language in Biophysics to students with different backgrounds.
Biophysics is a science that comprises theoretical plotting and models based on contemporary physicochemical conceptions. They mirror physical specificity of the molecular organization and elementary processes in living organisms, which in their turn form the molecular basis of biological phenomena. Presentation of a complete course in biophysics requires vast biological material as well as additional involvement of state-of-the-art concepts in physics, chemistry and mathematics. This is essential for the students to "perceive" the specific nature and peculiarity of molecular biological processes and see how this specificity is displayed in biological systems. This is the essence of the up-to-date biophysical approach to the analysis of biological processes.
Fundamentals of Biophysics offers a complete, thorough coverage of the material in a straightforward and no-nonsense format, offering a new and unique approach to the material that presents the appropriate topics without extraneous and unneeded filler material.
Andrey B. Rubin is a professor of biophysics at Lomonosov Moscow State University in the Department of Biophysics. Born in Russia, he is chair of the National Committee for Biophysics in the Russian Academy of Science. He was head of the Department of Biophysics at MSU, Governer of the Task Force on Education in Biophysics, and a member of the RAS Council on Space Biology and Biological membrane since 2005.?He has received many awards for his contributions to the science of biophysics, holds many patents and inventions, and authored of numerous papers. He is also on the editorial board of the journal, Biophysics, in the Russian language.
Contacts Program of Study General Education Requirements for the Biological Sciences Advanced Placement Credit Bachelor of Arts Degree in Biological Sciences Biology Track Specialization Programs in the Biological Sciences Interdisciplinary Biology Tracks Ecology and Evolution Track Global and Public Health Track Computational Biology Track Program Requirements for the Bachelor of Science in Biological Sciences Honors Research Opportunities Minor in Biological Sciences Grading and Academic Honesty Biological Sciences (BIOS) Courses Upper-level Elective Courses Big Problems Courses Specialized Courses Independent Study and Research Courses Graduate-Level Courses
Biology is the study of life, past and present. Our curriculum offers courses in many fields, from theoretical to experimental biology, and from molecular and genetic mechanisms underlying life to the complex interactions of organisms in ecosystems. As a major research institution, the University of Chicago focuses all courses in the Biological Sciences Collegiate Division on scientific reasoning, research, and discovery. The goals of the Biological Sciences curriculum are to give students (1) an understanding of currently accepted concepts in biology and the experimental support for these concepts, and (2) an appreciation of the gaps in our current understanding and the opportunities and tools available for new discoveries. A major in Biological Sciences can prepare students for careers in a wide range of areas, including health professions, basic or applied research in academia or industry, education, and policy related to human, animal, and planetary health.
Bachelor of Arts (BA): The BA is designed for students who wish to gain extensive training in the field of biology but also retain the flexibility to take elective courses outside the major. Scientific research is required for some tracks, but a thesis is not required to obtain a BA (although a thesis is required for some specializations; see details below).
Bachelor of Science (BS): The BS is designed for students who wish to delve more deeply into the field of their major through additional electives and completion of a BS thesis. Successful BS students will (1) learn how scientists design and conduct scientific experiments; (2) collect data as part of a research effort; (3) evaluate the strengths and weaknesses of that data; (4) interpret the data in the context of a specific scientific discipline; and (5) describe their work in a BS Thesis.
Bachelor of Arts/Bachelor of Science with Research Honors (Research Honors): Biology Research Honors is reserved for students who excel in the coursework of the major and have completed original research of high quality suitable for inclusion in a professional publication. Successful Research Honors students will (1) gain a scholarly understanding of a specific area of biology; (2) conduct scientific experiments, collect original data, analyze that data using appropriate statistics, and evaluate the strengths and weaknesses of the data; (3) interpret their findings in the context of their field; (4) describe their work in an Honors Thesis; and (5) present and defend their work in an oral presentation.
Bachelor of Arts/Bachelor of Science with Scholar Honors (Scholar Honors): Scholar Honors recognizes exceptional academic performance including submission and acceptance of a scholarly thesis.
A score of 4 or 5 on the AP Biology test will fulfill the general education requirement in the biological sciences ONLY for students who complete three quarters of the Advanced Biology Fundamentals Sequence.
Students with a score of 4 or 5 on the AP Biology test who complete the first three quarters of the Advanced Biology Fundamentals Sequence will be awarded three credits toward the Biological Sciences major and credit for completing the general education requirement in the Biological Sciences. This option is especially appropriate for students who plan to major in Biological Sciences and prepare for a career in research, but it is open to all qualified students including those planning a career in the health professions.
BIOS 20151 and BIOS 20153 fulfill the general education requirement in the biological sciences and are prerequisites for the rest of the courses in the Fundamentals Sequence. BIOS 20151 may be taken simultaneously with BIOS 20186.
Students who complete the Advanced Biology sequence but do not have a score of 4 or 5 on the AP Biology exam will need to take one additional course to fulfill the general education requirement in the Biological Sciences. Students should consult with the BSCD Senior Advisers (Megan McNulty, mmcn...@uchicago.edu; Chris Andrews, cand...@uchicago.edu) to select an appropriate course.
NOTE 1: The Biology Track does NOT require the third quarter of calculus. Students MUST take BIOS 20151 Introduction to Quantitative Modeling in Biology, and students in the Advanced Biology sequence MUST take BIOS 20236 Biological Dynamics. NO Mathematics courses may be substituted for these requirements.
NOTE 2: Students planning to apply to medical school should be aware of individual medical school admissions requirements and should tailor their program accordingly with the help of UChicago Careers in Healthcare.
Students completing the major in the Biology Track must take five upper-level courses (course numbers BIOS 21000 to 28999) to complete the Bachelor of Arts degree. These courses may be selected by the student or in consultation with the BSCD Senior Advisers (Megan McNulty, mmcn...@uchicago.edu; Chris Andrews, cand...@uchicago.edu).
If the student following the Biology Track chooses to focus their coursework in a specific area, they can complete a specialization. In this case, courses should be chosen in consultation with the specialization adviser (listed below).
Specializations represent recommended programs of study for students interested in one particular field within the biological sciences. Students who wish to complete a specialization should discuss their plans with the specialization director by Spring Quarter of their second year. Students may complete only one specialization. All courses must be taken for a quality grade in order to count toward a specialization.
NOTE: Beginning with the entering class of Autumn Quarter 2022, the Specializations in Global Health Sciences and Ecology and Evolution will no longer be available.* Beginning with the entering class of Autumn Quarter 2023, the Specialization in Quantitative Biology will no longer be available.** Students interested in focusing their major coursework in one of these fields can pursue an Interdisciplinary Biology track in Global and Public Health, Ecology and Evolution, or Computational Biology.
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