Scientist,biology 3yrs. experience

[Job Tharappel]

JOB C. THARAPPEL
Department of Nutrition and Food Science        E-mail :
jct...@ukcc.uky.edu
207 Funkhouser Bldg.               Tel     : (606) 257-2930 
(O)
University of  Kentucky               Fax     : (606) 257-3707
Lexington, KY 40506-0054               Tel     : (606) 323-7906 
(H)
 
OBJECTIVE
  To obtain an entry level research position in industry or academia.

PROFESSIONAL EXPERIENCE
UNIVERSITY OF KENTUCKY, LEXINGTON.
POST DOCTORAL FELLOW  (JUNE 1994 TO PRESENT)
  Planned and set up a lab for molecular biology protocols
  Optimized and validated molecular biology research protocols
  Designed and  cloned expression vectors
  Generated, analyzed and maintained transgenic mice lines
  Conducted EMSA studies on mammalian liver  samples

UNIVERSITY OF NAGPUR, INDIA.
RESEARCH ASSISTANT (JULY 1988 TO JULY 1993)
  Conducted neuronal tracing studies using neuronal tracers in fish brain
  Identified new neuronal pathways between hypothalamus and spinal cord in
fish
  Localized certain brain peptides in fish brain using immunohistochemical
methods
  Originated research materials, making selections for publication
  Designed, developed and validated research protocols
  Planned and coordinated laboratory activities

TECHNICAL EXPERTISE
MOLECULAR BIOLOGY                         CELL CULTURE
   Plasmid preparation                             Mammalian and bacterial
cell culture
   Isolation and purification of-                  Transient and stable
transfections
   plasmid and other DNAs                         Liver perfusion
   Restriction enzyme digestions                   Primary hepatocyte cell
culture
   Subcloning in plasmid vectors                   BIOCHEMICAL TECHNIQUES
   Designing and cloning  of                       Enzymatic assays
   expression vectors                              Immunohistochemistry (PAP &
Avidin-biotin)
   Generation, analysis and                        Various histological
staining procedures
   maintenance of transgenic mice lines            Neuronal Tracing
(Cobaltous-lysine & HRP)
   Southern, northern and western transfers        RESEARCH  EQUIPMENT
   Colony hybridization                            Agarose and polyacrylamide
gel equipments
   Electrophoretic mobility shift assays           Epiflourescent microscope
   DNA sequencing                               HPLC
   Probe labeling                                 Ultracentrifuge
   PCR                                        Cryostat
  COMPUTER SKILLS                           Microtome
   Dos/windows/Mac operating  systems              Microplate reader
   Word, Excel & Word Perfect                     Spectrophotometer &
Fluorometer
   Sigma Plot & Systat                            Liquid Scintillation
counter
   Plasmid artist & DNA Strider                    X-ray film processors
  COMMUNICATION SKILLS                    Radio analytical gel
scanners
   Strong oral and written communication skills. Small animal handling and
surgery

CITIZENSHIP
  USA

ACADEMIC RECORD
  Ph.D.     Neurobiology/Neuroanatomy, Nagpur University, Nagpur, India
(1988-1993)
  MS Zoology, Kerala university, Kerala, India (1981-1983)
  BS Biology, Kerala University, Kerala, India.(1977-1980)

PUBLICATIONS
1. Rao, P.D., Job, T.C. and Schreibman, M.P. (1993) Hypophysiotropic neurons
in the
     Hypothalamus of the Catfish Clarias batrachus (Linn.) A Cobaltous lysine
and HRP
     Study. Brain Behav. Evol., 42:24-38.
2.  Job, T.C. (1993) Hypothalamus and Hypophysis in catfish Clarias batrachus
(Linn.)
     Ph.D. thesis, Nagpur University, Nagpur, India.
3. Y. Li , Glauert H.P. , Job T.C. , and Spear B. T. (1997) Expression of
hydrogen peroxide
    generating enzyme fatty acyl coA oxidase activates
NFkB.Mol.Cell.Biol.(Manuscript submitted)
4. T.C. Job, Michael Cunningham, Spear B.T. , and  Glauert H.P. Species
difference in the
activation of hepatic NFkB by peroxisome proliferators (1997) (Manuscript
in Preparation)
CONFERENCES  AND WORKSHOPS
Participated in the symposium organized by Ohio Valley society of
Toxicology and presented a
poster "Species Difference in the Activation of Hepatic NFkB by Peroxisome
Proliferators".
    Participated in the symposium on ‘Neuroscience and Biotechnology’ and the
10th meeting of  the
    Indian Academy of Neurosciences (Dec. 12-14, 1990) and presented the paper
“Neuronal pathways.
    connecting  Hypothalamus and spinal cord in the catfish Clarias
batrachus.(Linn.)
    Participated in a workshop on the ‘Use of Scanning Electron Microscope in
Life Sciences
    at the Nagpur University, Nagpur.

AWARDS FELLOWSHIPS AND OTHER HONORS
  Associate member of  American Association for Cancer Research
  Training grant in Toxicology from the National Institute of Environmental
Health
  Sciences (06-27-94 to 06-26-96).
  Research paper (1993) illustration selected for the cover page of the
journal, “Brain
  Behavior and Evolution”.
  Awarded first prize for the best research paper presentation, 1993, Nagpur
University
  Nagpur, India
  Awarded a junior research fellowship in 1986 by the University Grants
Commission
  (UGC) India.
  Awarded a senior research fellowship in 1990 by the UGC (India)
  B.Sc. degree  passed in first division (Equivalent to ‘A’ in the letter
grade system)

REFERENCES
1. Howard P. Glauert Ph.D.         2.Brett T. Spear Ph.D.     3.Kwaku Addo
Ph.D.
    Professor                      Associate Professor          Associate
Professor &
    Nutrition & Food Science       Microbiology & Immunology    Director of
Food Science Program
    222 Funkhouser Bldg.           UK Chandler Medical Center   Nutrition &
Food Science
    University of Kentucky         University of Kentucky       University of
Kentucky
    Lexington, KY 40506           Lexington Ky 40506           Lexington KY
40506-0054
    Tel. (606) 257-7789            Tel. (606) 257-5167   Tel. (606)
257-7784
    E-mail: hgla...@pop.uky.edu   E-mail: bsp...@pop.uky.edu   E-mail:
Kad...@pop.uky.edu