Eppendorf Centrifuge 5417

[Alayna Rother]

TheEppendorf 5417C is a non-refrigerated bench top centrifuge with reliable performance and easy operation. The Eppendorf 5417C can centrifuge Eppendorf micro test tubes at up to 16400 rpm in a compact footprint. This benchtop centrifuge runs quietly (

For the NON-CHILLER version, remove the four side screws, and the entire housing should come right off. If not, turn it upside down and note the rusty crust that's gluing the metal frame to the plastic! Slide a knife, or pry to break the bond. Then invert again, lift the housing carefully and reach inside to remove the ribbon cable from the PCB (pinch the plastic connector sides to release.) There are also two ground cables and the release-string to remove. (Also note, if you want to remove the main PCB, must unscrew the four screws on the BOTTOM of the centrifuge, not the four screws on the PCB itself.

I've now seen more than one 5417C Eppendorf lab centrifuge with the same problem: sudden death after power had been turned off (or, it died right after a local power outage.) No fuses blown. The LCD remains blank. Sometimes the LEDs for "OPEN" and "SOFT" start blinking rapidly. The +5VDC is missing, or giving crazy pulsesand never rising up to 5V.

The switching supply for the LCD assembly is repeately restarting: a giant 2Hz sawtooth on the input to the +5VDC regulator. Simple cure: on the main PCB inside, replace a tiny 22uF 35V capacitor labeled "C824," next to IC UC2844AD (switching supply controller chip.) This fixes an apparent problem of slow-aging of the "boot circuit" in the switching supply. The 22uF has dried out and decreased below 5uF,so during power-up, the DC supply cannot wake up before the boot-voltage decreases below 10V. (Yeah right, "simple" cure. Only have to disassemble the entire unit to get to the bottom of the circuit board. SEE PHOTOS BELOW.)

NOTES AND TRICKS:

Sometimes these units will start up if you unplug the LCD/buttons cable, turn on power, then plug the cable back in. But in older units this requires removing the plastic case! (In newer units the LCD assembly pops out: remove the little side-plug hidden below, and pry.SEE PHOTOS BELOW.)To access the LCD/Buttons (newer models only,) find the white plastic plug in the ledge below the LCD, over on the left. Remove it, TURN POWER OFF!!!, then use a tiny philips screwdriver or a 1/16" allen wrench to push out that entire side of the LCD assembly: insert the metal shaft 1" or more, then pull the tip forward. The whole thing pops out. (Do this with power off, of course!) Also, when removing the entire centrifuge cover, the ground-strap on the LCD can easily be unscrewed through here.To get to the main PCB without removing the entire cover and case, first remove the motor's round plastic cover inside the rotor-well, plus the four large leveling feet on the bottom of the unit. Unhook the white string at the bottom left corner of the unit (the rotor release cord,) then tilt the entire motor assembly upwards. Don't pull on the wires inside. Also, don't forget about the "rotor detector" that was under the round motor cover (easy to let it slip inside and not re-installed later.)If removing the entire door, take a photo of the shaft/springs, otherwise you'll forget how they mesh together. And, DON'T INSTALL THE COVER BACKWARDS.If removing the entire case, don't forget to replace the string and white plastic plug, otherwise nobody can manually release the cover latch.If removing the entire case, yes, you must first disassemble the door hinge assembly and remove the steel shaft (remove the snap-ring so the shaft can slide slightly. Don't lose that snap ring!)

The Eppendorf Centrifuge 5417 C is a non-refrigerated benchtop centrifuge are can be used in research and routine laboratories in the biosciences and in the fields of medicine and industry. Eppendorf Micro Test Tubes can be centrifuged at 20800 rcf (14000 rpm). It is possible to attain an rcf of up to 25000 using a special 24-position rotor. For special applications a swing-bucket rotor is available with 8 positions for centrifuging Eppendorf Micro Test Tubes at a maximum rotational speed of 10500 rpm (11700 rcf). PCR tubes can be centrifuged in a rotor which can accommodate six 8-tube strips.

High performance spins up to 25000 x g for quick and efficient pelletization. This model offers unsurpassed safety reliability and ease of use. The Eppendorf 5417C Microcentrifuge is designed for use on the lab bench or in a cold room and can accommodate a variety of microcentrifuge rotors to extend application range.

With the unit powered off, hold down the Soft and Time Up keys, turn the power switch on while keeping those keys held down, and keep holding them down until the display reads SE followed by a number (possibly two digits).

You should then see two sets of numbers. The left hand set will be the chamber temperature reading, which should be a sensible number (like room temp). If it is three digits long, the last digit will be tenths. If you see anything other than that your sensor is bad or has a bad connection. Turn the power switch off to exit the service mode.

Held the soft and tme up keys down while powering up the fuge, still got an error 20 flashing. If this is a chamber temp sensor error, where is the sensor located and how can it be tested. I have the fuge apart! Is it mounted in the lid and connected by blue and red wires?

I'm guessing they are still available, it may even still be used on newer models. You'd have to contact Eppendorf. The old Brinkmann p/n is 5811643.000; Eppendorf will translate that into something starting with 022.

Are there any markings on what's left of the stainless steel sheath? It's a two-wire (red and blue) sensor so probably a standard T-type thermocouple - pretty much universal in refrigeration applications. You can probably get one from a local instrumentation place, like WIKA, or on line from Omega.

I opened up another 5417R and Ta da! It has the temperature probe in the bottom of the chamber in front of the spindle. The one I am working on has the temp probe in the lid I kid you not. Both fuges-same model number but different revisions for sure.

Contacted eppendorf and they no longer carry the thermistor for the 5417 s/n 05210, as it is a 3 wire sensor assume it is a thermistor, unfortunately the part number on it partially obliterated or for sure I would second source it. Main difference between the two 5417rs s that the older unit has a separate housing for the compressor and is sealed to the front housing with foamy tape while the newer unit has a one piece housing with a plastic lid over the compressor section.

The Eppendorf 5417C Centrifuge is a reliable microprocessor controlled microcentrifuge that spins up to 16,400 rpm (fixed angle rotor) with consistent quiet operation. The 5417C features automatic rotor detection, which automatically calibrates to rotor specifications. Ample for cold room usage, the 5417C can used with multiple Eppendorf rotors, such as a 30 place, 24-place, swinging bucket 8-place, or six 8-tube strips.

The Heraeus Multifuge X3R is a compact benchtop centrifuge.

The user-friendly control panel makes it easy to pre-set the speed, RCF value, running time, temperature, and running profile (acceleration and braking curves). You can choose between the display of speed and RCF or the entry mode. With the help of the PULSE key, you can also centrifuge a sample for just a few seconds.

The Eppendorf Centrifuge 5417 R is a refrigerated bench-top centrifuge. Eppendorf Micro Test Tubes can be centrifuged at 20800 rcf (14000 rpm). It is possible to attain an rcf of up to 25000 using a special 24-position rotor. For special applications a swing-bucket rotor is available with 8 positions for centrifuging Eppendorf Micro Test Tubes at a maximum rotational speed of 10500 rpm (11700 rcf). PCR tubes can be centrifuged in a rotor which can accommodate six 8-tube strips.

The Tycho NT.6 verifies protein quality by looking at the structural integrity (or foldedness) of a protein. Changes in the intrinsic fluorescence of the sample are measured as a thermal ramp is applied. Changes in the fluorescence signal indicate transitions in the folding state of the protein. The Tycho can be used to study the stability of protein samples in different buffers or at different pH values. The measurement is completed in three minutes and uses a capillary tube to hold the sample.

The Biacore T200 is a surface plasmon resonance instrument which can be used to characterize molecular interactions between biomolecules ranging in size from ions to viruses. The Biacore software provides a range of tools for kinetic analyses. These analyses can be performed using a multicycle approach (many samples against one ligand or when different ligands are immobilized), or alternatively, using fast, single cycle kinetics.

The Lonza 4D Nucleofector is a system designed for efficient transfection of various cell types. It comprises the 4D-Nucleofector X Unit, facilitating transfection of diverse cell numbers. The Core Unit controls the system for transfecting cell lines and primary cells.

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These high performance microcentrifuges spin up to 25,000g and offer up to 30-place capacity for high throughput. Centrifuges incorporate reinforced steel rotor housing and double lid locks. The maintenance-free, brushless induction motor reaches maximum speed within 12 seconds for quick separation and recovery of samples. Model 5417C has an integrated fan to prevent excessive heat generation in the rotor chamber. Rotors are autoclavable.

Operating parameters of time, speed (or RCF), soft start/stop and momentary spins are entered on a touch-sensitive pad. The timer includes a hold function for longer spins. The soft start/stop function slows the acceleration and deceleration rate for delicate samples or filter tubes.

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