error occur while perform the assembly creation using trinity.

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Usha

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May 10, 2024, 9:02:48 PMMay 10
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i am run this command.
/mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/Trinity --seqType fa --left left.fasta --right right.fasta --max_memory 5G --CPU 3 --output Trinity_output.dnv

it show the error.
Error, pairs.K25.stats is empty.  Be sure to check your fastq reads and ensure that the read names are identical except for the /1 or /2 designation. at /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/util/insilico_read_normalization.pl line 921.
Error, cmd: /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/util/insilico_read_normalization.pl --seqType fa --JM 5G  --max_cov 50 --min_cov 1 --CPU 3 --output /mnt/e/DenovoRNAseq/Trinity_output.dnv/insilico_read_normalization   --max_pct_stdev 10000  --left /mnt/e/DenovoRNAseq/left.fasta --right /mnt/e/DenovoRNAseq/right.fasta --pairs_together --PARALLEL_STATS   died with ret 512 at /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/Trinity line 2581.
        main::process_cmd("/mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/util/insilico_"...) called at /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/Trinity line 3127
        main::normalize("/mnt/e/DenovoRNAseq/Trinity_output.dnv/insilico_read_normaliz"..., 50, ARRAY(0x5654290162a0), ARRAY(0x5654290162b8)) called at /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/Trinity line 3074
        main::run_normalization(50, ARRAY(0x5654290162a0), ARRAY(0x5654290162b8)) called at /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/Trinity line 1294

kindly help me to solve this issue .
thank you.
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