Insilico read normalization

[Robert García Mendoza]

Hi, I have these error messages, when I made a test with short reads trinity worked well. But when I run my dataset around 100M reads a got these:
 Error, cmd: /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl --seqType fq --JM 24G --max_cov 200 --min_cov 1 --CPU 4 --output /mnt/z/RNAseqMCF7/MCF_7/trinity/trinity_out/norm_for_read_set_1 --max_CV 10000 --left /mnt/z/RNAseqMCF7/MCF_7/trinity/MCF_7_ctrl_1_R1.fq.gz --right /mnt/z/RNAseqMCF7/MCF_7/trinity/MCF_7_ctrl_1_R2.fq.gz --pairs_together --PARALLEL_STATS died with ret 7424 at /home/dna/anaconda3/envs/trinity/bin/Trinity line 2919.
 main::process_cmd("/home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insi"...) called at /home/dna/anaconda3/envs/trinity/bin/Trinity line 3472
 main::normalize("/mnt/z/RNAseqMCF7/MCF_7/trinity/trinity_out/norm_for_read_set_1", 200, ARRAY(0x5613ce8ee138), ARRAY(0x5613ce8f1a08)) called at /home/dna/anaconda3/envs/trinity/bin/Trinity line 3389
 main::run_normalization(200, ARRAY(0x5613ce6bbf10), ARRAY(0x5613ce6bbf28)) called at /home/dna/anaconda3/envs/trinity/bin/Trinity line 1450

bash: line 1: 11345 Killed                  /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 3 --DS > left.fa.K25.stats
Thread 3 terminated abnormally: Error, cmd: /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25  --num_threads 3  --DS  > left.fa.K25.stats died with ret 35072 at /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl line 807.
Error, thread exited with error Error, cmd: /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25  --num_threads 3  --DS  > left.fa.K25.stats died with ret 35072 at /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl line 807.

bash: line 1: 11344 Killed                  /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 3 --DS > right.fa.K25.stats
Thread 4 terminated abnormally: Error, cmd: /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25  --num_threads 3  --DS  > right.fa.K25.stats died with ret 35072 at /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl line 807.
Error, thread exited with error Error, cmd: /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25  --num_threads 3  --DS  > right.fa.K25.stats died with ret 35072 at /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl line 807.

Error, 2 threads errored out at /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl line 1011.
Error, cmd: /home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insilico_read_normalization.pl --seqType fq --JM 24G  --max_cov 200 --min_cov 1 --CPU 6 --output /mnt/z/RNAseqMCF7/MCF_7/trinity/trinity_out/norm_for_read_set_1 --max_CV 10000  --left /mnt/z/RNAseqMCF7/MCF_7/trinity/MCF_7_ctrl_1_R1.fq.gz --right /mnt/z/RNAseqMCF7/MCF_7/trinity/MCF_7_ctrl_1_R2.fq.gz --pairs_together  --PARALLEL_STATS   died with ret 7424 at /home/dna/anaconda3/envs/trinity/bin/Trinity line 2919.
        main::process_cmd("/home/dna/anaconda3/envs/trinity/opt/trinity-2.15.2/util/insi"...) called at /home/dna/anaconda3/envs/trinity/bin/Trinity line 3472
        main::normalize("/mnt/z/RNAseqMCF7/MCF_7/trinity/trinity_out/norm_for_read_set_1", 200, ARRAY(0x55dc48b6e410), ARRAY(0x55dc48b70778)) called at /home/dna/anaconda3/envs/trinity/bin/Trinity line 3389

        main::run_normalization(200, ARRAY(0x55dc4893c240), ARRAY(0x55dc4893c258)) called at /home/dna/anaconda3/envs/trinity/bin/Trinity line 1450