Assembling a 127Gb .fastq file (500M reads)

[justin]

Hi all, I'm trying to perform a de novo transcriptomic assembly, my R1 and R2 paired-end read files are 127Gb each, 500M reads each. I tried doing the assembly on UseGalacxy but it did not work due to "too much data for galaxy server backend". I am now attempting this on my university's high performance computing clusters. It is not working so far, I am still trying to make it work. Has anyone tried this? Any tips? Thank you.