Facing the issue while using RSEM

[Usha]

I am a new to bioinformatics ,  when i performing DenovoRNAseq while performing abundance estimate using RSEM

i got an error kindly help me to solve this issue.

i am using this command.

 /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/util/align_and_estimate_abundance.pl --seqType fq --transcript /mnt/e/DenovoRNAseq/Trinity_output.dnv/Trinity.fasta --left trim_Treated1_1.fastq --right trim_Treated1_2.fastq --est_method RSEM --aln_method bowtie --trinity_mode --prep_reference --output_dir /mnt/e/DenovoRNAseq/Trinity_output.dnv/RSEM_Treared1

i got a error like this

Error, cmd: rsem-calculate-expression  --paired-end   -p 4    --no-bam-output --bam bowtie.bam.for_rsem.bam /mnt/e/DenovoRNAseq/Trinity_output.dnv/Trinity.fasta.RSEM RSEM  died with ret: 65280 at /mnt/e/DenovoRNAseq/TOOLS/Trinityrnaseq-v2.6.6/util/align_and_estimate_abundance.pl line 790.

thank you.