Histology Slide Bank
Jomega Gibson
Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.
This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at umich.edu.
The histology service offers routine and specialized histology services including grossing, embedding, tissue processing, sectioning and staining, whether routine H&E or specialized. It operates on frozen and FFPE tissue. It also offers tissue micro array construction service and expertise, and an experimental immunohistochemistry/ immunofluorescence service. Last year, it processed more than 13,000 blocks and an excess of 161,000 sections were generated. The service is known for its high quality, quick turn around time and dedicated personnel.
In order to address the many challenges associated with traditional tumor banking's utility, and to reduce barriers to research, in the molecular era, we developed in 2002 the Molecular Platform (next generation banking).
The macromolecule platform is a research platform designed to facilitate, Columbia University wide and beyond, cancer research and foster collaboration by evaluating systematically the procured frozen tumors in our tumor bank. This is done by providing a set of thoroughly characterized frozen tumors and normal tissue, corresponding DNA/RNA (macromolecules extracted, quantified and assessed for quality, purity, diagnosis, size and stored), corresponding tissue micro-arrays, whole section banks of formalin fixed slides corresponding to cases, frozen tissue for Western blots/IHC, database (linked clinical/pathological and basic science characteristics) as well linkage to related cases (primary removed piece meal, recurrent and metastatic cases), professional services and expertise in tissue based analysis (extraction, storage, expression, and immuno and molecular genetic characterization).
We also provide molecular biology services on a project-specific level to investigators operating on fresh/frozen or FFPE tissues or analytes prepared by our next generation biobanking service. These include:
Lastly, during the past few years we introduced full cycle project management service, which allows researchers to send a cohort list (patients) and the facility will carry out and return biomarker analysis - managing the full cycle of pre-analyte retrieval, analyte isolation, biomarker analysis, reporting, and storage. As an example, following receipt of a cohort of patients and their controls we retrieve the corresponding H&E slides and blocks from the Department of Pathology, match them to frozen tissue, review H&E slides for desired lesions (usually a specific cancer type), make either TMA or slide banks (for IHC, 10X 10 micron slide bank for RNA or DNA extractions or protein), perform IHC/extract DNA/RNA/protein (for array based expression, RNAseq, copy number, methylation studies, RPPA), evaluate IHC, and report out results.
The Molecular Pathology Shared Resource uses the iLab Core Management System for service requests, billing, and policies. Before you can request services, you must log into iLab and be approved as a lab member.
Auto bright field whole slide scanning system producing sharp images of up to 40x magnification with 100 seconds average scanning time for 20x magnification. Up to 384 slides could be loaded with autoloader per loading. Standard slide size (26x76 mm)
Welcome to Norecopa, Norway's 3R platform!
A global Map of 3R Centres and Networks
The PREPARE Guidelines for Planning Animal Research and Testing
International Webinars and Meetings Calendar
Creating a Culture of Care
Alternatives to dissection
Lessons learned from COVID-19
Noah's Arkive is a collection of slide images contributed by veterinarians all over the world, formed to share teaching materials. Type of record: Web Pages. Category: Haematology, Histology, Medicine, Parasitology and Pathology
Noah's Arkive is a collection of slide images contributed by veterinarians all over the world, formed to share teaching materials. The images include gross lesions, histopathology, normal histology, cytology and hematology, parasitology, poisonous plants, animals with/without clinical signs, schematics, radiographs, electron micrographs and techniques. The Arkive is now run by the Davis/Thompson Foundation at no charge at this website. Future plans include allowing submission of photos from the public, as well as allowing rating of photos to highlight particularly good depictions of diseases.
Comments & References: For the advancement of veterinary and comparative pathology. Suitable for instructors needing images to illustrate lessons or presentations, and for persons studying for exams.
The WVU Neurobiology and Anatomy Department has developed a virtual microscopy web site that features a bank of digitized whole slide histological specimens that permits the user to scan and manipulate magnification in a zoom-like fashion. Rather than viewing isolated images, the virtual microscope offers a more progressive and engaging mode of teaching that preserves the interpretive and diagnostic aspects of traditional microscopy.
When you choose Fisherbrand slides for your histology applications, you have access to an unmatched breadth of options and flexibility to meet your needs. Made from Swiss white glass, Fisherbrand microscope slides are selected for uniform quality and reliable clarity, with precision-ground edges and consistent dimensional tolerances. Their excellent adhesion and hydrophilic properties offer dependable, high-quality, consistent performance with instrumentation. In addition, each slide meets stringent quality control standards to ensure that your lab operations and service levels can be reliably maintained.
When you invest in Sakura Finetek products, you can depend on high quality products and expect support, minimal downtime and quick, reliable service which are critial to you, your laboratory and your patients.
The Tissue-Tek Lab Aid Filing Cabinet System comes with two different filing drawer sections and can be stacked in any combination. The histology slide filing cabinet system allows convenient storage and retrieval of microscopic slides or transparencies. The 25 mm drawer section is designed to hold 26 x 76 mm microscopic slides. Each section contains 14 drawers, with a capacity of 465 slides per drawer. The 50 mm drawer section is designed to hold 50 x 50 mm transparencies or 50 x 76 mm microscopic slides. The drawer section is complete with 7 drawers, which will accommodate 271 transparencies per drawer. The slide filing cabinet system is constructed of welded steel for durability and requires 19 x 19 inches (48.3 x 48.3 cm) of floor space. Slide backstops easily attach to the inside of the drawers to hold slides in place when the drawer is not full. A slide liner (spring) is also available to hold slides upright in the histology slide filing cabinet during the drying process.
Pictured right: The tissue bank in the LaViolette Lab and Neuro-Oncology Brain Bank. The Huron slide scanner is on the bench next to a Windows work station equipped with Huron software. The iMac workstation shown is a hub for a 70Tb drive, which stores the raw slide data. On the right are whole brain samples from the Neuro-Oncology Brain Bank.
Pictured left: The Neuro-Oncology Brain Bank is located on the 4th floor of the MACC Fund Research Building and currently houses over 65 whole brain samples from brain cancer patients and is the largest such resource in the country.
The Neuro-Oncology Brain Bank lab also houses a rapidly expanding database that contains both the clinical imaging from each patient, clinical histories, and digital histology obtained from each case. This resource is housed locally within the lab on servers with over 120Tb of storage. Additional MCW resources such as the Research Computing Center (RCC) are also utilized for high-throughput computing and long-term storage. As this database grows, future resources will be necessary to continue to support it, which will include additional storage, and additional high-performance computer systems.
The Neuro-Oncology Brain Bank is equipped with four 3D printers, three Makerbots and one Form Labs Form2. The Makerbots are used to print the prostate and brain slicing jigs used for aligning pathology with imaging. We also 3D print models for surgical planning purposes.
The LaViolette Lab recently purchased a Huron slide scanner capable of scanning three large format histology slides at once at 40X resolution. This resource is located in the tissue bank room and is being used for digitizing both H&E stained slides and immunohistochemistry slides for molecular markers.
The LaViolette Lab and Neuro-Oncology Brain Bank is currently funded by the National Institute of Health (NIH), the National Cancer Institute (NCI), through an academic-industrial partnership with Novocure Inc. and through a pilot grant funded by philanthropic funds earmarked for neurooncology research at Froedtert and MCW. Past grants have come from the MCW Research Affairs Committee, the American Brain Tumor Association, and the American College of Radiology Imaging Network.
Our Novocure funded project is focused on a new treatment. Therapy with tumor treatment fields (TTFields) has recently been FDA approved for the treatment of newly diagnosed glioblastoma due to a recent clinical trial that showed improvement in progression free survival and overall survival compared to standard therapy. TTFields also have a role in the recurrent glioblastoma treatment where it has demonstrated equal efficacy to second-line chemotherapy also has been shown to tumor progression and improve overall survival. Though preclinical studies are ongoing, glioblastoma patients who have undergone TTField therapy have not yet been assessed at autopsy to determine both the pathological signature of TTField treatment, and the pattern of failure. This study will determine how the underlying pathological signatures of tumors treated with TTFields differ from those nave to TTFields by comparing tumor tissue at autopsy. We will also assess the imaging to determine whether the TTFields effect on cell division varies spatially.
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