ORFs in genomic coordinate

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Renee Salz

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Jan 21, 2021, 9:41:27 AM1/21/21
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I am trying to convert the longest orf gff3 file (produced by LongOrfs) from transcript coordinates to genome coordinates. Is the script 'util/cdna_alignment_orf_to_genome_orf.pl' intended for this? I found the explanation a bit insufficient, I couldn't find whether this is the correct file for this purpose and what exactly the inputs should be...

'usage: ../../../TransDecoder-TransDecoder-v5.5.0/util/cdna_alignment_orf_to_genome_orf.pl cdna_orfs.genes.gff3 cdna_genome.alignments.gff3 cdna.fasta [DEBUG]'

what are those 3 files?


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