Magellan Power Mcr-ii Manual
Melissa Russian
Easy selection of one of the four preset configurations for a specific application segment provides a fast solution to solve your research problems. The fully configurable platform also gives you the freedom to personalize your own Spark reader. The Spark is also upgradeable so that you can add modules at any time in the future.
A good wavelength ratio accuracy in A260/280 or A260/230 is crucial for DNA/RNA purity checks, ensuring repeatable and reproducible data, but this parameter is not specified for most systems on the market.
For convenience and optimal data quality, the NanoQuant Plate is the only low volume plate on the market that is 100 % calibration free, maintaining reliable performance while minimizing demands on your time. This innovative tool truly is a prerequisite for sensitive, reliable and easy DNA/RNA quantification in your laboratory.
This unique combination of optical components ensures maximum sensitivity for weak luminescent signals and allows attenuation of high signals. The result is an industry-leading dynamic range of 109, combined with exceptionally low detection limits for glow and flash luminescence.
Flawless performance of Alpha Technology assays requires high quality instruments. Spark combines a high power laser light source with an innovative filter wheel and dedicated photon counting PMT for ultra-sensitive detection of Alpha assays.
Simple and cost-efficient cell imaging built directly into a multimode reader. The Spark cell imaging module consists of a LED brightfield illumination, a 4x objective lens, a CMOS 1.3 megapixel camera and a robust laser-based autofocus system, delivering performance to rival an automated microscope.
Your life as a cell biologist revolves around keeping your cells happy, no matter the time or day. Take back control of your time and keep your cells happy by automating your workflows. Spark brings together the capabilities of an automated incubator, a microplate reader and a cell imager in one, offering new possibilities for you and your lab.
Spark is equipped with precise temperature control to enable uniform heating of your microplate. To help ensure even temperature distribution, the microplate chamber is thermally isolated from the optics, guaranteeing stable and precise temperature regulation for your assays.
Generating growth curves with bacteria or yeast also requires effective plate shaking. Based on years of experience and knowledge of working with cell lines in microplate readers, Spark was specifically engineered for long-term shaking and incubation of micro-organisms.
SparkControl combines automation of long-term kinetic assays with advanced features such as kinetic conditioning, remote operation and Open Kinetic, providing a hands-off solution for complex experimental set-ups.
A multimode reader is frequently used for multiple applications by various users. Long-term live cell kinetics assays can block the reader for an entire day, limiting overall lab throughput. SparkControl offers an efficient solution: Open Kinetic. This feature allows you to pause and resume kinetic experiments, enabling additional measurements to be performed throughout long-term studies. This frees up the instrument for other users, eliminating one of the major bottlenecks of shared lab equipment, without compromising your valuable research.
Automated monitoring of metabolic activity and differentiation of human mesenchymal stem cells
Real-time analysis of stem cell proliferation during differentiation
Walk-Away Monitoring of Cytotoxicity, Viability and Apoptosis
Temperature plays a crucial role on biochemical assays using enzymes derived from non-mammalian organisms. For example, bioluminescence assay systems require optimal temperatures between 20 and 25 C. These assays are usually highly temperature sensitive, with even a 1 C increase in temperature leading to a 10 % increase in luminescence output. Luminometers or multimode readers that cannot stably control temperature in this critical range will therefore generate irreproducible data.
Multiple users require multiple experimental conditions. A major bottleneck for microplate readers is waiting for an instrument to reach the desired temperature. Heating up the measurement chamber is easy, but what if you have to wait for it to cool down?
It typically takes over 60 minutes for a reader to cool down from 37 C to room temperature through passive cooling, costing you valuable time and disrupting your busy schedule. Imagine the time delays and decrease in productivity you could avoid in a multi-user lab by just shortening that cool-down window.
Spark brings together a unique set of features intended to ease this strain and create new workflow possibilities for your laboratory, reducing hands-on time while producing high quality data for each and every sample.
Whether you are looking to design long-term experiments that work around your schedule, attempting to shrink sample volumes to reduce costs, or trying gain access to multiple instruments on a tight budget, Spark gives you control over your priorities.
As scientists, we consider ourselves data-driven. Whether your research is centered in academia or industry, your next discovery will rely on your data. The ability to generate reliable, reproducible and high quality data should be a prerequisite for all laboratory instruments.
Spark is designed to address almost every detail of the most demanding assays and applications, delivering technologies that seamlessly work together to allow you to generate accurate and consistent data, each and every day.
The new SparkControl 3.2 software includes multiple features improving the overall user experience. For example, benefit from the new label-free cell counting functionality to simplify your cell-based assay workflow and save valuable time.
SparkControl excels in handling routine or complex workflows. Whether you need to quantify a nucleic acid or automate an injection at a specific absorbance value, SparkControl works for you, freeing up more time for your research.
Open Kinetic allows you to pause and resume kinetic reads. In the case of long-term experiments or experiments with long reading intervals, this feature enables other users to perform separate applications between kinetic measurements.
High resolution 3D scanning provides simultaneous excitation and emission scans, giving you the ability to quickly identify changes in the spectral properties of fluorescent probes and characterize unknown fluorescent samples.
SparkControl features a special Optimal Read (OR) function designed to improve cell-based measurements. OR delivers increased sensitivity for enhanced FI bottom reading by performing multiple measurements on spatially separated spots arrayed across each well.
Integrated injectors give you valuable dispensing capabilities, ideal for high sensitivity applications such as flash luminescence. Unique heating and stirring capabilities reduce the risk of precipitation.
The NanoQuant Plate allows parallel quantification and analysis of as little as 2 l of up to 16 nucleic acid or protein samples. The unique design of the NanoQuant Plate, with a quartz optic for each sample, negates the need for calibration and guarantees consistent performance.
The novel ValitaTITER assay provides a very accurate and cost-effective solution for the detection and quantification of IgG and Fc-containing proteins directly in cell culture supernatants In this application note, we show the optimal protocol for performing IgG/Fc-protein quantification using ValitaTITER assay kits with the Spark and the Infinite 200 PRO multimode plate readers.
The Optimal Read function for fluorescence bottom measurements is designed to enable uniform well illumination during excitation and whole-well emission for 6-to 384-well plates. Performing multiple measurements on spatially separated spots arrayed across each well ensures that even heterogeneous cell layers can be effectively and reproducibly analyzed. This application note shows that the Optimal Read function produces the most reliable results for the analysis of adherent cell types, giving the highest well-to-well uniformity and best sensitivity for cell-based applications.
This application note describes the successful implementation of AlphaScreen on the Spark multimode reader, as well as the performance of the instrument with regards to measurement sensitivity, uniformity and typical read times.
This application note describes the implementation of the AlphaLISA technology on the Spark multimode reader and its use for the detection of human immunoglobulin G (IgG) using the AlphaLISA IgG Assay Kit.
Assaying cells in an appropriate window of time after a compound-mediated cytotoxic event can be extremely challenging. This note describes the automation for kinetic monitoring of cytotoxicity and viability using three Promega assays for viability, cytotoxicity and apoptosis assessment with the Spark multimode reader.
Cell migration is a central process in the development and maintenance of multicellular organisms, with tissue formation during embryonic development, wound healing and immune responses all requiring the orchestrated movement and assembly of cells.
Fast and reliable nucleic acid quantification and purity checks are important daily routine applications for many laboratories, driven by the widespread use of next generation sequencing and other high throughput nucleic acid analysis techniques. Many methods require rapid, accurate analysis of small sample volumes to keep pace with downstream processes.This technical note shows the optimized Spark measurement settings, as well as comparative results, for the commonly used fluorescence-based DNA quantification assays evaluated in this study.
The Spark reader is equipped with Tecan's unique Fusion Optics, which combine the benefits of filters and MCRs in a single multimode reader. The Spark can be equipped with filters, Quad4 Monochromators or a combination of both using the ingenious Spark Fusion Optics.
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