Subsequences as separate proteoforms?

[SR]

Hello In a recent Toppic result I found the  two proteoforms having different proteoform IDs
In both cases they are from the same Protein( Uniprot sp|O43169|CYB5B_HUMAN)  and one sequence is just longer than the other. 

Should such sub sequences be reported as a single proteoform?


M.(S)[Acetyl]GSMATAEASGSDGKGQEVETSVTYYRLEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGHSSDAREMLKQ.Y

and

M.(S)[Acetyl]GSMATAEASGSDGKGQEVETSVTYYRLEEVAKRNSLKELWLVIHGRVYDVTRFLNEHPGGEEVLLEQAGVDASESFEDVGHSSDAREMLKQYYIGDIHPSDLKPESGSKDPSKNDTCKSCWAYWILPIIGAVLLGFLYRYYTSESKSS.

[James Fulcher]

They should be considered different proteoforms. Here are a few references that describe what constitutes a proteoform: https://www.nature.com/articles/nmeth.2369 and https://www.nature.com/articles/s41592-019-0573-x

Bests,

James

[liuxia...@gmail.com]

James, 

Thank you for answering the question. 

Xiaowen