Proteoform Mass Errors in TopPIC

[Qianjie Wang]

Hello Xiaowen,

May I ask if there is a way to extract all mass errors from the preteoforms?

Thank you!

Best,

Qianjie

[liuxia...@gmail.com]

Hi, Qianjie, 

You can compute errors in  precursor masses by comparing the values in the columns "precursor mass" and "proteoform mass" in proteoform identification files: ..._proteoform.tsv.  

TopPIC does not report errors in fragment ions. To compute these errors, you need to compute theoretical fragment masses and compare them with fragment masses in MS/MS spectra. 

Best, 

Xiaowen