I think the Nikon N-STORM software specifies a base level of 100 A/D for this camera, but I found it to be a bit lower on mine. The 12.48 value is the A/D gain before EM amplification, corresponding to the 5.1x converter gain (I you use 1x it will be around 5 times lower).
Hope this helps,
Great work with the ThunderSTORM! Thanks first for all the work you did.
This post really helped me.
We use the same camera (Andor ixon+ DU897) and we got the manufacturers information that the baseline AD count is 200. If insert this baseline count we get the same warning message:
'Camera base level is set higher than values in the image!'
Christophe said something interesting, the N-STORM Nikon software (NIS Advanced) puts the AD count to 100. We couldn't find this setting in the software. Does anyone know where to find it?
I know this is an old post, still, I would be glad if someone had a hint.
pixel size [nm]: 160
photoelectrons per A/D count: 16.2
base level [A/D counts]: 200
EM gain: 250