Re: Biology: Gel Electrophoresis, III-6, III-7

[The Home Scientist]

Yes, you should be able to re-run the gels as long as you keep them immersed in running buffer.

How large are the gels, how much voltage were you using, and how long did you run the gels?

On Thursday, February 21, 2013 12:40:54 PM UTC-5, fox...@gmail.com wrote:

We tried the gel electrophoresis and could not get any migration of the dyes. I tried both agar and non-flavored gelatin. Also tried moving the electrical leads to the gel container instead of the buffer container. No luck. My fellow teacher had the same non-results. We're on well water; I used mostly purchased spring water, but will try it again with distilled water. Any other suggestions?

Also, I saved the gels that I made on Monday and they seem in fairly good shape. Can I use them again?

Thanks,

Mitzi Price

Virginia

[The Home Scientist]

Incidentally, it'd be a good idea to "clear" the gels before running them again by running them with no analyte to pull any ions present in the well water out of the gels and into the surrounding bath of running buffer.

[j yeardly]

Ok this may seam dumb did you have the power  the correct way round  
the + is normally away from the wells and the - near
see
http://www.ncbe.reading.ac.uk/NCBE/MATERIALS/DNA/baseunit.html
For good info
also an Idea for a PSU rather than batts
I run 8 of these tanks at a time with no problems
 
Joe