Hi Kanhaiya,
How do you mean? An ENI can only be attached to an EC2 instance after it's been created, as far as I'm aware.
You can attach an ENI to an instance in different states (running, stopped, maybe a few others), but I don't think it's possible to attach one before the instance hits the created state.
(If it's the Launch Wizard you're referring to, that's basically just getting hold of all of the values for the arguments to pass to the relevant EC2 APIs, so whilst it's on multiple pages, it's not actually doing anything with the information you provide until click Launch at the end.)
Does that help out?
Andrew