001 Micron Pen

[Latanya Hariri]

HelloI started using imageJ a few months ago, so I am not an expert on it yet. My doubt is: I have a quantity of images which express cells. I used the default settings on imageJ to calculate the surface area of these cells and then I figured out that I should have measured these in squared microns instead squared pixels.

I must transform the surfaces, perimeter and mean diameter of Feret of these cells of pixels into micrometers in the table excel.

Attached is the image processed by imageJ (1024x768 pixels) ready with magnification 10 and an image for the micrometer slide ready at the same magnification.

if at all possible, can you help me convert these parameters in squared micron instead squared pixels?

if no, how can I calibrate imageJ before processing this image?

Thank you

Since 40-50 micron objects are the smallest things visible to the naked human eye, these extremely small dimensions can be hard to wrap your head around. So, we decided to use some great laser microscope images to put size in perspective.

The eye of a fruit fly is also 70 microns across. But the eye itself isn't the smallest unit: each eye is made up of 760-unit eyes that are about 5 microns in diameter each! This is similar to the placement tolerance Benchmark achieves in fully automated microelectronic assembly.

Moving to more familiar territory, our own bodies, our cells are on a similar scale to the placement precision required in microelectronics. A human skin cell is 20-40 microns across, and a white blood cell is approximately 30 microns. Red blood cells get closer to microelectronics tolerances with an average width of 6-8 microns.

To find things that are one micron, you really have to get sub-cellular. A human mitochondrion is 1 micron across but 7 microns long. Small bacteria can be as small as .5 microns. This is comparable to the placement precision Benchmark achieves in semi-automated microelectronic assembly for applications such as lidar, free space optics, medical imaging, and photonics communications and computing applications.

All of this just goes to show that sometimes the smallest things can make the biggest difference. At only one micron wide our mitrochondria are tiny, but we wouldn't survive without them. Similarly, 1 micron precision in microelectronics placement is key to the functioning of these high-tech products.

Jennifer McAlpine is the Product Marketing Manager at Benchmark, communicating the value of Benchmark's product realization services. Prior to joining Benchmark, she was a Foreign Service Officer with the U.S. Department of State, serving in U.S. Embassies in Ireland, Gabon, Algeria, and Guinea. She holds an MBA from W. P. Carey School of Business at Arizona State University and a BA in Economics from the University of Minnesota.

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