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Lance T. Yonkos

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Mar 29, 2015, 8:21:54 PM3/29/15
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…so I assume all is well.  I’ll be in early tomorrow if any last minute things need to be sorted out.

Taylor Throwe

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Mar 29, 2015, 8:25:54 PM3/29/15
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"Collection, handling, and testing of all species adhered to protocols approved by the University of Maryland Institutional Animal Care and Use Committee (IACUC). "

You said in a comment about this, I’ll get IACUC number to put here.



On Sun, Mar 29, 2015 at 8:21 PM, Lance T. Yonkos <lyo...@umd.edu> wrote:

…so I assume all is well.  I’ll be in early tomorrow if any last minute things need to be sorted out.

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Taylor T. Throwe
University of Maryland
Robert H. Smith School of Business

Taylor Throwe

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Mar 29, 2015, 8:28:13 PM3/29/15
to team...@googlegroups.com, Lance T. Yonkos
Oops, didn't mean to send that yet, also there was this...

Note to self: Look up Dr. Aga’s standards mix about this ". These samples were then augmented  with 100 µL of an Deuterated Estrogen Standard Mix (EE2-d) provided by Dr. Aga of the University of Buffalo to provide a reference for determining extraction/elution efficiency and percentage recovery of unknown analytes."

and this..

We bought our own yeast strain. I’ll confirm the source tomorrow. This is probably where we should cite the original Sansevrino BLYES paper. 
 
Did you find the yeast strain source?

Thanks for all your edits/help! You're the best mentor ever! Have a good night!

Taylor Throwe

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Mar 29, 2015, 9:58:47 PM3/29/15
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---------- Forwarded message ----------
From: Lance T. Yonkos <lyo...@umd.edu>
Date: Sunday, March 29, 2015
Subject: Haven't heard from anyone...
To: tthrowe-contact <taylor...@gmail.com>


Answers to questions below.  Anything else?

 

1.       IACUC approved:  Protocol # R-13-16

 

2.       Change to read:   “These samples were then augmented with a deuterated estrogen internal standard (supplied by Dr. Diana Aga of the University at Buffalo) to allow determination of extraction/elution efficiency and percentage recovery of unknown analytes.”

 

3.       We received the BLYES yeast strain (Saccharomyces cerevisiae BLYES ) from 490 BioTech, Inc.

 

Citation is:

Sanseverino J,Gupta RK, Layton AC, Patterson SS, Ripp SA, Saidak L, et al. Use of Saccharomyces cerevisiae BLYES expressing bacterial bioluminescence for rapid, sensitive detection of estrogenic compounds. Appl Environ Microb 2005;71:445560.

 

 

On Sun, Mar 29, 2015 at 8:25 PM, Taylor Throwe <taylor...@gmail.com> wrote:

"Collection, handling, and testing of all species adhered to protocols approved by the University of Maryland Institutional Animal Care and Use Committee (IACUC). "

You said in a comment about this, I’ll get IACUC number to put here.

 

Image removed by sender.

 

On Sun, Mar 29, 2015 at 8:21 PM, Lance T. Yonkos <lyo...@umd.edu> wrote:

…so I assume all is well.  I’ll be in early tomorrow if any last minute things need to be sorted out.

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Taylor T. Throwe

University of Maryland

Robert H. Smith School of Business



 

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Taylor T. Throwe

University of Maryland

Robert H. Smith School of Business

Lance T. Yonkos

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Mar 29, 2015, 10:12:03 PM3/29/15
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????? Taylor - no message came with email.

Shivani Patel

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Mar 29, 2015, 11:30:41 PM3/29/15
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Hi Dr. Yonkos,

Have you been able to thoroughly look at the Discussion section? We were hoping to get your eyes on that section before sending the thesis out tomorrow.

Shivani
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