Any tips on speeding up output from HDF5?

[P St. Amand]

Any tips on speeding up output from HDF5?

For large projects, outputting a VCF or Hapmap from the HDF5 is quite slow. Does anyone know of ways to speed up this process? Below are examples of how I am creating VCFs and Hapmaps from HDF5 files. Any tips would be greatly appreciated!


/usr/local/bin/tassel-5-standalone/run_pipeline.pl -Xms80g -Xmx190g -fork1 -h5 HDF5/$Study\_productioHapMap_noKO.h5 -filterAlign -filterAlignMinFreq $MAF -filterAlignRemMinor -export ./hapmap/$Study.vcf -exportType VCF -runfork1 > ./logs/VCFFromHDF5.log

/usr/local/bin/tassel-5-standalone/run_pipeline.pl -Xms80g -Xmx190g -h5 HDF5/$Study\_productioHapMap_noKO.h5 -filterAlign -filterAlignMinFreq $MAF -filterAlignRemMinor -export hapmap/$Study.hmp.txt -exportType Hapmap > ./logs/HapmapFromHDF5.log

[Brandon Monier]

A couple of things to try out are:

• not include allele depth (e.g., -noDepth) if you don't need it,
• split and export by chromosome (e.g., run_pipeline.pl -Xmx190g -SplitHDF5ByChromosomePlugin -i HDF5/$Study.h5) - this may avoid cache issues that you could experience with exporting a whole genome's worth of variants.

Best,

Brandon M.