How to enzyme deatils for HaeIII

[Dr Mathavan M]

Hi there,

I new to this WGS analysis, I start working on command line mode. My samples has treated with HaeIII enzyme, but this enzyme is not updated in GBSEnzyme.jar file. I don't understand the meaning of the highlighted

[MSPI] name = MspI

initialCutSiteRemnant = CGG

likelyReadEnd = CCGG,CCGAGATCGG

readEndCutSiteRemnantLength = 3

Anyone please help me how to edit this for  enzyme HaeIII

else if (enzyme.matches("(?i)ecot22[i1]")) {

theEnzyme = "EcoT22I";

initialCutSiteRemnant = new String[]{"TGCAT"};

likelyReadEnd = new String[]{"ATGCAT", "ATGCAAGAT"}; // full cut site (from partial digest or chimera) or common adapter start

readEndCutSiteRemnantLength = 5;