I am running the GBS pipeline using Tassel 4 on our Linux server which runs Scientific Linux. Tassel 4 uses Java 1.7, which runs well for all plugins in the GBS pipeline.
Since Tassel 5 is now under current development and also gives some more options, I am very much interested in running Tassel 5. I have Tassel 5.2.10, hence the most recent update, I believe. I can run the FastqToTagCountPlugin, MergeMultipleTagCountPlugin, align the reads tags with BWA, convert the sam file using the SAMConverterPlugin, but then the FastqToTBTPlugin gives me only 0 tags as ouput. There is some heap.size error for java appearing, but I have my heap size at 40GB, which should be sufficient for 570 samples. Since it runs in Tassel4, I assume its a bug in Tassel 5 or its based on the Java version we are using. We have the Open JDK1.8 installed. Do you use the Oracle Java 1.8 version for development, or is that error due to another issue.
Before we will change the servers to Oracle Java, we wanted to make sure it will run afterwards.
Thanks
Willmar