Raw sequence data (Illumina MiSeq 16S rRNA sequencing, V4 hypervariable region) can be found at -archive.org/datasets/EGAD00001004106. The HELIUS data are owned by the Amsterdam University Medical Centers, located at the AMC in Amsterdam, The Netherlands. Any researcher can request the data by submitting a proposal to the HELIUS Executive Board as outlined at , accessed on 28 March 2022, by email: heliusco...@amsterdamumc.nl. The HELIUS Executive Board will check proposals for compatibility with the general objectives, ethical approval and informed consent forms of the HELIUS study. There are no other restrictions to obtaining the data and all data requests will be processed in the same manner.
The HELIUS data are owned by the Amsterdam University Medical Centers, location AMC in Amsterdam, The Netherlands. To allow sharing of microbiome data collected in HELIUS with (inter)national researchers, 16 s rRNA sequence analysis has been stored at the European genome-phenome archive (EGA; accession code EGAD00001004106). This requires that access needs to be granted, also because the HELIUS data are stored with relevant phenotypical variables. Access is granted to all researchers affiliated with an internationally recognized research institution who request to use the HELIUS data within the EGA context, after having signed the data transfer agreement (Supplementary file 1). Any researcher can request the data by submitting a proposal to the HELIUS Executive Board as outlined at , by email: heliusco...@amsterdamumc.nl. The HELIUS Executive Board will check proposals if they do not conflict with ethical approvals and informed consent forms of the HELIUS study. The reads from the validation cohort are available from NCBI under the NCBI BioProject accession number PRJEB28687. All code used for generating the main figures and performing the statistical analyses are shown in Supplementary file 2. The custom-made Python script including the machine learning analysis is available at GitHub ( _Generations).
Data Availability: The HELIUS data are owned by the Amsterdam University Medical Centers, location AMC in Amsterdam, The Netherlands. Any researcher can request the data by submitting a proposal to the HELIUS Executive Board as outlined at , by email: heliusco...@amsterdamumc.nl. The HELIUS Executive Board will check proposals for compatibility with the general objectives, ethical approvals and informed consent forms of the HELIUS study. There are no other restrictions to obtaining the data and all data requests will be processed in the same manner.
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