Metabolite Quantification _ Siemens csi_30 and 135 without water unsupressed file

[Sai Manohar]

Dear Tarquin Community !

 

Greetings.

 

I am Sai Manohar , PhD student working on Huntington's disease from India. I am new to MR spectroscopy and also To Tarquin. I need your valuable suggestions with respect to multivoxel MR spectroscopy analysis.

 

Objective:

 

1. To quantify NAA, Cho, Cr, Lac, m-Ins, Glu, Gln, Ala, Asp levels in the caudate, putamen and thalamus region of patients/controls and do relative statistics
2. To show heat map of metabolite distribution in different regions of the brain

 

Drawbacks:

 

1. Acquired data using 1.5 T machine
2. Dont have the water unsuppressed  file

 

Following are the parameters of the data:

 

Machine: Siemens 1.5T MRI

Sequence: csi_se

 

Acquisition Parameters:

1. 16 x 16 voxel grid
2. TE 30 and TE 135 (csi_30 and csi_135); TR: 1500 ms
3. 1 measurement with 4 averages.
4. Voxel size: 10x10x15mm

I have fitted csi_135 control subject file using the default parameters in Tarquin.  I was getting lot of noise. ( attached the peak fitting screenshot)

 

Help Required:

1. What are the parameters to use in Data parameters, Preprocessing, Fitting, Postprocessing.
   
2. How to check the quality of my peaks and reliability of my results.

 

I request the community to kindly help me and give me your valuable suggestions.

 

Thank you so much,

Sai