Are TALENs more efficient when targeted towards the start of a gene

[Elaine O'Connor]

Have TALENs greater efficiency when targeted towards the start of a gene rather than the end?

[rem...@gmail.com]

On Tuesday, July 29, 2014 6:02:48 AM UTC-4, Elaine O'Connor wrote:
> Have TALENs greater efficiency when targeted towards the start of a gene rather than the end?

The specificity does not change. However, if you want to disrupt your gene of choice, it would be best to target the TALEN to the start of a gene (i.e. exon 1). That way when a mutation happens the higher the possibility that the indels will throw you protein out frame early and cause a knock out rather than a dominant negative protein.