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TAL Effectors Discussion Forum

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Welcome to the TAL Effectors Discussion Forum. Feel free to post your questions or thoughts regarding the TALE and TALEN technology.

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2/16/21

I am happy that my message has been delivered to you, since my effort to contact you was delayed due

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I am happy that my message has been delivered to you, since my effort to contact you was delayed due

2/16/21

Neville Sanjana, elpheg...@gladstone.ucsf.edu2

8/30/16

Revamped TALE website and TALE reference sequence online tool

Dear Neville, Thank you for updating the website. It seems something with the reference sequence

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Revamped TALE website and TALE reference sequence online tool

Dear Neville, Thank you for updating the website. It seems something with the reference sequence

8/30/16

Kimberly Wooster

7/5/16

Colony PCR or send for sequence

Hello, I am on my 4th try with the Golden Gate protocol and trying to assemble my TALENs. Assuming

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Colony PCR or send for sequence

Hello, I am on my 4th try with the Golden Gate protocol and trying to assemble my TALENs. Assuming

7/5/16

megb...@gmail.com, woos...@umn.edu2

7/5/16

Problems with golden gate/voytas

This happened to me as well. If there are any Golden Gate experts who could help, this would be very

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Problems with golden gate/voytas

This happened to me as well. If there are any Golden Gate experts who could help, this would be very

7/5/16

dav.pi...@gmail.com

1/14/16

TALEN injection into mouse zygotes : plasmid vs rna

Hi, I am new to TALEN injection into moue zygotes. I saw a lot of publications with talen rna

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TALEN injection into mouse zygotes : plasmid vs rna

Hi, I am new to TALEN injection into moue zygotes. I saw a lot of publications with talen rna

1/14/16

grot...@gmail.com, … Neville Sanjana5

10/15/15

wrong link to TALE reference sequence generator!

Hi all, Our apologies for the extended downtime of the online TALE tool for generating reference

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wrong link to TALE reference sequence generator!

Hi all, Our apologies for the extended downtime of the online TALE tool for generating reference

10/15/15

Alvaro Sequeida

9/8/15

Partial TALEN binding

Hi everyone! I know that TALEN specificity is very high, but anyone has references or has experienced

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Partial TALEN binding

Hi everyone! I know that TALEN specificity is very high, but anyone has references or has experienced

9/8/15

lina W, … natha...@gmail.com6

7/29/15

Low yield of plasmid DNA

Sorry I forgot about that detail. I did grow them up at 37˚C overnight but my range has still only

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Low yield of plasmid DNA

Sorry I forgot about that detail. I did grow them up at 37˚C overnight but my range has still only

7/29/15

lacr...@gmail.com, Le Cong2

7/10/15

single nucleotide base change

Hi Tuang, If you could make some silent or non-deleterious mutations to your repair oligo, then you

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single nucleotide base change

Hi Tuang, If you could make some silent or non-deleterious mutations to your repair oligo, then you

7/10/15

live2l...@gmail.com, Le Cong3

6/28/15

Can I validate target gene expression by transfecting pAAV-hSyn-TALE-VP64/SIDX4-2A-EGFP constructs into Neuro2A cells?

So, just to follow up on the use of N2A cells for pAAV-hSyn-TALE-VP64/SIDX4 2A-EGFP construct

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Can I validate target gene expression by transfecting pAAV-hSyn-TALE-VP64/SIDX4-2A-EGFP constructs into Neuro2A cells?

So, just to follow up on the use of N2A cells for pAAV-hSyn-TALE-VP64/SIDX4 2A-EGFP construct

6/28/15

6/19/15

Re: Abridged summary of taleffectors@googlegroups.com - 1 update in 1 topic

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

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Re: Abridged summary of taleffectors@googlegroups.com - 1 update in 1 topic

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

6/19/15

6/18/15

Re: Abridged summary of taleffectors@googlegroups.com - 2 updates in 2 topics

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

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Re: Abridged summary of taleffectors@googlegroups.com - 2 updates in 2 topics

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

6/18/15

manohara...@gmail.com

6/9/15

TAL Effector gene identification

Hi All, Does any one know how to identify TAL effector genes in xanthomonas genome? Thanks &

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TAL Effector gene identification

Hi All, Does any one know how to identify TAL effector genes in xanthomonas genome? Thanks &

6/9/15

5/17/15

Detecting Mutations

I was wondering how others have been detecting their mutations from their TALEN. I have been trying

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Detecting Mutations

I was wondering how others have been detecting their mutations from their TALEN. I have been trying

5/17/15

brian...@gmail.com, Le Cong2

3/21/15

trouble ligating hexamers into pAAV-TALE backbones

Hi Brian, These plasmids should have double BsaI sites for cloning in the repeat monomers as other

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trouble ligating hexamers into pAAV-TALE backbones

Hi Brian, These plasmids should have double BsaI sites for cloning in the repeat monomers as other

3/21/15

binnu gangadharan

3/20/15

sub cloning of pTALEN_v2

Hi pTALEN_v2 talen vectors dont have any GFP/RFP to visualize the transfection and the hygromycin

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sub cloning of pTALEN_v2

Hi pTALEN_v2 talen vectors dont have any GFP/RFP to visualize the transfection and the hygromycin

3/20/15

binnu gangadharan, … Neville Sanjana6

3/20/15

PTALEN_V2 vector

Dear Nivelle few questions: which bacterial resistance do pTALEN_v2 vectors have? In the addgene site

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PTALEN_V2 vector

Dear Nivelle few questions: which bacterial resistance do pTALEN_v2 vectors have? In the addgene site

3/20/15

1/20/15

Is TALENs tranfection a stable transfection

Hi all, I have transfected a set of TALENs arms in a squamous cell carcinoma cell line, the selection

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Is TALENs tranfection a stable transfection

Hi all, I have transfected a set of TALENs arms in a squamous cell carcinoma cell line, the selection

1/20/15

christiaa...@gmail.com, … hyasuda...@gmail.com6

1/8/15

TALEN efficiency: electroporation versus "normal transfection"

Hi, Christian; Please try to use NEPA21 electroporator. This is new innovation from Japanese company.

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TALEN efficiency: electroporation versus "normal transfection"

Hi, Christian; Please try to use NEPA21 electroporator. This is new innovation from Japanese company.

1/8/15

shrikant...@gmail.com

12/29/14

History of TALE

Sir plz send me history of TALE, ie who discovered it,developed it etc. thank you

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History of TALE

Sir plz send me history of TALE, ie who discovered it,developed it etc. thank you

12/29/14

Alvaro Sequeida

10/23/14

There's a minimum of assays to publish results from a mutation using TALEN?

Hi everyone, I've mutated an exon of a gene from rainbow trout, but I want to know if there's

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There's a minimum of assays to publish results from a mutation using TALEN?

Hi everyone, I've mutated an exon of a gene from rainbow trout, but I want to know if there's

10/23/14

ibrahi...@gmail.com, … wangz...@126.com34

10/19/14

Testing TALEN function in vitro

Hi Steph, I am Zhuo, I want to use your protocol to test my TALEN plasmids in vitro, would you to

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Testing TALEN function in vitro

Hi Steph, I am Zhuo, I want to use your protocol to test my TALEN plasmids in vitro, would you to

10/19/14

asahi...@gmail.com2

10/15/14

http://www.addgene.org/21280/

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http://www.addgene.org/21280/

10/15/14

patrick...@gmail.com

9/19/14

Creating short TALENs (<10 RVDs) using Voytas lab golden gate cloning kit

Dear All, I'm trying to create TALENs which have multiple binding sites throughout the

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Creating short TALENs (<10 RVDs) using Voytas lab golden gate cloning kit

Dear All, I'm trying to create TALENs which have multiple binding sites throughout the

9/19/14

Elaine O'Connor, rem...@gmail.com2

9/11/14

Are TALENs more efficient when targeted towards the start of a gene

On Tuesday, July 29, 2014 6:02:48 AM UTC-4, Elaine O'Connor wrote: > Have TALENs greater

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Are TALENs more efficient when targeted towards the start of a gene

On Tuesday, July 29, 2014 6:02:48 AM UTC-4, Elaine O'Connor wrote: > Have TALENs greater

9/11/14

ansor...@gmail.com

7/23/14

Hi there My name is Ansor. We have knockouted a phyB gene in cotton via RNAi technology. And

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Hi there My name is Ansor. We have knockouted a phyB gene in cotton via RNAi technology. And

7/23/14

Danioivanio, pdh...@gmail.com2

6/11/14

Minimize off-targets and improve Cripr/cas specificty

hey Ivan, you will get more answers if you post in the Genome Engineering CRISPR forum. but the short

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Minimize off-targets and improve Cripr/cas specificty

hey Ivan, you will get more answers if you post in the Genome Engineering CRISPR forum. but the short

6/11/14

Erik Nielson, pdh...@gmail.com3

6/11/14

PlasmidSafe vs NEB Endonuclease V

Yes, I increasingly like KAPA Hifi... On Thursday, June 5, 2014 12:53:52 PM UTC-4, Erik Nielson wrote

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PlasmidSafe vs NEB Endonuclease V

Yes, I increasingly like KAPA Hifi... On Thursday, June 5, 2014 12:53:52 PM UTC-4, Erik Nielson wrote

6/11/14

6/3/14

Any success in generating KI mice through microinjecting TALEN mRNA + dsDNA ?

Dear List, We've been trying to introduce EGFP into a mouse gene loci through TALEN by co-

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Any success in generating KI mice through microinjecting TALEN mRNA + dsDNA ?

Dear List, We've been trying to introduce EGFP into a mouse gene loci through TALEN by co-

6/3/14

elph...@gmail.com, … banni...@gmail.com24

5/20/14

Deletion using two pairs of TALENs

Hi All, Lots of comments so far, but just wanted to add that we often use two pairs of TALENs

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Deletion using two pairs of TALENs

Hi All, Lots of comments so far, but just wanted to add that we often use two pairs of TALENs

5/20/14

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