TAL Effectors Discussion Forum - Google Groups

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Welcome to the TAL Effectors Discussion Forum. Feel free to post your questions or thoughts regarding the TALE and TALEN technology.

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Aug 25

Daniel will be coming around again

Hey neighbors! Daniel will be coming around again to clean our air vents and dryer vents. He did an

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Daniel will be coming around again

Hey neighbors! Daniel will be coming around again to clean our air vents and dryer vents. He did an

Aug 25

Aug 18

Daniel will be coming around again

Hey neighbors! Daniel will be coming around again to clean our air vents and dryer vents. He did an

unread,

Daniel will be coming around again

Hey neighbors! Daniel will be coming around again to clean our air vents and dryer vents. He did an

Aug 18

Aug 7

William will be coming around again

Hey neighbors! William will be coming around again to clean our air vents and dryer vents. He did an

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William will be coming around again

Hey neighbors! William will be coming around again to clean our air vents and dryer vents. He did an

Aug 7

Jun 27

MIKE IS COMING IN OUR AREA!

Hey neighbors! I've been dealing with poor airflow and dust buildup throughout my home, so I

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MIKE IS COMING IN OUR AREA!

Hey neighbors! I've been dealing with poor airflow and dust buildup throughout my home, so I

Jun 27

2/16/21

I am happy that my message has been delivered to you, since my effort to contact you was delayed due

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I am happy that my message has been delivered to you, since my effort to contact you was delayed due

2/16/21

Neville Sanjana, elpheg...@gladstone.ucsf.edu2

8/30/16

Revamped TALE website and TALE reference sequence online tool

Dear Neville, Thank you for updating the website. It seems something with the reference sequence

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Revamped TALE website and TALE reference sequence online tool

Dear Neville, Thank you for updating the website. It seems something with the reference sequence

8/30/16

Kimberly Wooster

7/5/16

Colony PCR or send for sequence

Hello, I am on my 4th try with the Golden Gate protocol and trying to assemble my TALENs. Assuming

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Colony PCR or send for sequence

Hello, I am on my 4th try with the Golden Gate protocol and trying to assemble my TALENs. Assuming

7/5/16

megb...@gmail.com, woos...@umn.edu2

7/5/16

Problems with golden gate/voytas

This happened to me as well. If there are any Golden Gate experts who could help, this would be very

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Problems with golden gate/voytas

This happened to me as well. If there are any Golden Gate experts who could help, this would be very

7/5/16

dav.pi...@gmail.com

1/14/16

TALEN injection into mouse zygotes : plasmid vs rna

Hi, I am new to TALEN injection into moue zygotes. I saw a lot of publications with talen rna

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TALEN injection into mouse zygotes : plasmid vs rna

Hi, I am new to TALEN injection into moue zygotes. I saw a lot of publications with talen rna

1/14/16

grot...@gmail.com, … Neville Sanjana5

10/15/15

wrong link to TALE reference sequence generator!

Hi all, Our apologies for the extended downtime of the online TALE tool for generating reference

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wrong link to TALE reference sequence generator!

Hi all, Our apologies for the extended downtime of the online TALE tool for generating reference

10/15/15

Alvaro Sequeida

9/8/15

Partial TALEN binding

Hi everyone! I know that TALEN specificity is very high, but anyone has references or has experienced

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Partial TALEN binding

Hi everyone! I know that TALEN specificity is very high, but anyone has references or has experienced

9/8/15

lina W, … natha...@gmail.com6

7/29/15

Low yield of plasmid DNA

Sorry I forgot about that detail. I did grow them up at 37˚C overnight but my range has still only

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Low yield of plasmid DNA

Sorry I forgot about that detail. I did grow them up at 37˚C overnight but my range has still only

7/29/15

lacr...@gmail.com, Le Cong2

7/10/15

single nucleotide base change

Hi Tuang, If you could make some silent or non-deleterious mutations to your repair oligo, then you

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single nucleotide base change

Hi Tuang, If you could make some silent or non-deleterious mutations to your repair oligo, then you

7/10/15

live2l...@gmail.com, Le Cong3

6/28/15

Can I validate target gene expression by transfecting pAAV-hSyn-TALE-VP64/SIDX4-2A-EGFP constructs into Neuro2A cells?

So, just to follow up on the use of N2A cells for pAAV-hSyn-TALE-VP64/SIDX4 2A-EGFP construct

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Can I validate target gene expression by transfecting pAAV-hSyn-TALE-VP64/SIDX4-2A-EGFP constructs into Neuro2A cells?

So, just to follow up on the use of N2A cells for pAAV-hSyn-TALE-VP64/SIDX4 2A-EGFP construct

6/28/15

6/19/15

Re: Abridged summary of taleffectors@googlegroups.com - 1 update in 1 topic

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

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Re: Abridged summary of taleffectors@googlegroups.com - 1 update in 1 topic

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

6/19/15

6/18/15

Re: Abridged summary of taleffectors@googlegroups.com - 2 updates in 2 topics

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

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Re: Abridged summary of taleffectors@googlegroups.com - 2 updates in 2 topics

Automatic Vacation Response: Auto-Reply: Hello, I am out of the country until June 30 with only

6/18/15

manohara...@gmail.com

6/9/15

TAL Effector gene identification

Hi All, Does any one know how to identify TAL effector genes in xanthomonas genome? Thanks &

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TAL Effector gene identification

Hi All, Does any one know how to identify TAL effector genes in xanthomonas genome? Thanks &

6/9/15

5/17/15

Detecting Mutations

I was wondering how others have been detecting their mutations from their TALEN. I have been trying

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Detecting Mutations

I was wondering how others have been detecting their mutations from their TALEN. I have been trying

5/17/15

brian...@gmail.com, Le Cong2

3/21/15

trouble ligating hexamers into pAAV-TALE backbones

Hi Brian, These plasmids should have double BsaI sites for cloning in the repeat monomers as other

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trouble ligating hexamers into pAAV-TALE backbones

Hi Brian, These plasmids should have double BsaI sites for cloning in the repeat monomers as other

3/21/15

binnu gangadharan

3/20/15

sub cloning of pTALEN_v2

Hi pTALEN_v2 talen vectors dont have any GFP/RFP to visualize the transfection and the hygromycin

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sub cloning of pTALEN_v2

Hi pTALEN_v2 talen vectors dont have any GFP/RFP to visualize the transfection and the hygromycin

3/20/15

binnu gangadharan, … Neville Sanjana6

3/20/15

PTALEN_V2 vector

Dear Nivelle few questions: which bacterial resistance do pTALEN_v2 vectors have? In the addgene site

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PTALEN_V2 vector

Dear Nivelle few questions: which bacterial resistance do pTALEN_v2 vectors have? In the addgene site

3/20/15

1/20/15

Is TALENs tranfection a stable transfection

Hi all, I have transfected a set of TALENs arms in a squamous cell carcinoma cell line, the selection

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Is TALENs tranfection a stable transfection

Hi all, I have transfected a set of TALENs arms in a squamous cell carcinoma cell line, the selection

1/20/15

christiaa...@gmail.com, … hyasuda...@gmail.com6

1/8/15

TALEN efficiency: electroporation versus "normal transfection"

Hi, Christian; Please try to use NEPA21 electroporator. This is new innovation from Japanese company.

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TALEN efficiency: electroporation versus "normal transfection"

Hi, Christian; Please try to use NEPA21 electroporator. This is new innovation from Japanese company.

1/8/15

shrikant...@gmail.com

12/29/14

History of TALE

Sir plz send me history of TALE, ie who discovered it,developed it etc. thank you

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History of TALE

Sir plz send me history of TALE, ie who discovered it,developed it etc. thank you

12/29/14

Alvaro Sequeida

10/23/14

There's a minimum of assays to publish results from a mutation using TALEN?

Hi everyone, I've mutated an exon of a gene from rainbow trout, but I want to know if there's

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There's a minimum of assays to publish results from a mutation using TALEN?

Hi everyone, I've mutated an exon of a gene from rainbow trout, but I want to know if there's

10/23/14

ibrahi...@gmail.com, … wangz...@126.com34

10/19/14

Testing TALEN function in vitro

Hi Steph, I am Zhuo, I want to use your protocol to test my TALEN plasmids in vitro, would you to

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Testing TALEN function in vitro

Hi Steph, I am Zhuo, I want to use your protocol to test my TALEN plasmids in vitro, would you to

10/19/14

asahi...@gmail.com2

10/15/14

http://www.addgene.org/21280/

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http://www.addgene.org/21280/

10/15/14

patrick...@gmail.com

9/19/14

Creating short TALENs (<10 RVDs) using Voytas lab golden gate cloning kit

Dear All, I'm trying to create TALENs which have multiple binding sites throughout the

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Creating short TALENs (<10 RVDs) using Voytas lab golden gate cloning kit

Dear All, I'm trying to create TALENs which have multiple binding sites throughout the

9/19/14

Elaine O'Connor, rem...@gmail.com2

9/11/14

Are TALENs more efficient when targeted towards the start of a gene

On Tuesday, July 29, 2014 6:02:48 AM UTC-4, Elaine O'Connor wrote: > Have TALENs greater

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Are TALENs more efficient when targeted towards the start of a gene

On Tuesday, July 29, 2014 6:02:48 AM UTC-4, Elaine O'Connor wrote: > Have TALENs greater

9/11/14

ansor...@gmail.com

7/23/14

Hi there My name is Ansor. We have knockouted a phyB gene in cotton via RNAi technology. And

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Hi there My name is Ansor. We have knockouted a phyB gene in cotton via RNAi technology. And

7/23/14

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