Retrieving RPKM, FPKM, AND TPM values

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R adele

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Apr 17, 2020, 12:01:02 PM4/17/20

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Hello I did pair-ended RNA-seq, how can I convert my read count numbers generated by feature counts into RPKM, FPKM, and TPM values ? I'm currently using subread1.5.3

Thanks

Wei Shi

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Apr 19, 2020, 8:01:43 PM4/19/20

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Subread does not offer functions to calculate these values. You will have to use other tools to calculate them or you can calculate them yourself. The rpkm() function in Bioconductor package edgeR can help you to calculate rpkm and fpkm values.

R adele

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Apr 29, 2020, 12:56:20 AM4/29/20

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ok thanks

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