Explanation about "Mean Fst value"

[hbkaya]

Hi all,

I ran Structure analysis and it separated genotypes into two groups. I obtained Fst values for these two groups. 

As far as I know, in order to show divergence and heterogeneity within predefined subpopulations,  Fst (F statistic) is measured. The Fst, a measure of population differentiation by estimating the correlation of alleles within the same subpopulation, is obtained from STRUCTURE Software."

Also, Structure calculates the mean Fst values, not pairwise Fst values. In this case, it gives us the differentiation within the same subpopulation.

According to this information, I added the information below in my thesis.

"According to the allelic frequency in subpopulations, Fst values of marker loci were calculated to show the differentiation. The Fst values were about 39% (Fst=0.39) in Group 1 and 13% (Fst=0.13) in Group 2. The Fst values suggested a high level of heterozygosity for genotypes within subpopulations. The differentiation among genotypes in Group 1 was higher than that in Group 2."

I checked the previous questions and answers in this group however I couldn't find any good explanation about Fst calculation. I also checked the manual. I couldn't find any article that used Fst values from Structure Software. 

I would like to ask you whether this information is correct or not?

Any suggestion is welcome.

Thanks.

Best,

Betul

[Vikram Chhatre]

For a discussion about Mean_Fst output from Structure, see this thread:

https://groups.google.com/forum/#!topic/structure-software/76BeGT8-Rjw/discussion

As for your FST values, they seem very high.  Is that to be expected in your study species?

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