error in running the tool.

[Saikat Banerjee]

hello everyone here,
I am trying to run Strelka for my tumor-normal data.  and my commands are

saikat@saikat-OptiPlex-3046:~/bio-tools/strelka_workflow-1.0.15/bin$ ./configureStrelkaWorkflow.pl --normal= /media/saikat/Mafia\ Boy/CSIR\ IGIB/test_1_NL/DYP26_NL_aln_lane1_sorted_marked_dup_RG_added_recal_applied.bam --tumor= /media/saikat/Mafia\ Boy/CSIR\ IGIB/test_1_lesion_lane1/DYP26_L_l1_S1_L001_aln_sorted_Marked_RG_added_recal_applied.bam --ref= /media/saikat/Mafia\ Boy/CSIR\ IGIB/vitiligo\ analysis/resources%2Fbroad%2Fhg38%2Fv0%2FHomo_sapiens_assembly38.fasta --config= /media/saikat/Mafia\ Boy/CSIR\ IGIB/strelka-var-calling/config.ini --output-dir= /media/saikat/Mafia\ Boy/CSIR\ IGIB/strelka-var-calling/./my-analysis-L-vs-NL

but it does not work and gives this output in the terminal

Option normal requires an argument
Option tumor requires an argument
Option ref requires an argument
Option config requires an argument
Option output-dir requires an argument
Usage:
    configureStrelkaWorkflow.pl --tumor FILE --normal FILE --ref FILE
    --config FILE [options]

    This script configures the strelka workflow for somatic variant calling
    on matched tumor-normal BAM files. The configuration process will
    produce an analysis makefile and directory structure. The makefile can
    be used to run the analysis on a workstation or compute cluster via
    make/qmake or other makefile compatible process.

Arguments:
    --tumor FILE
        Path to tumor sample BAM file (required)

    --normal FILE
        Path to normal sample BAM file (required)

    --ref FILE
        Path to reference genome fasta (required)

    --config FILE
        Strelka configuration file. Default config files can be found in
        ${STRELKA_INSTALL_ROOT}/etc/ for both ELAND and BWA alignments.
        (required)

Options:
    --output-dir DIRECTORY
        Root of the analysis directory. This script will place all
        configuration files in the analysis directory, and after
        configuration all results and intermediate files will be written
        within the analysis directory during a run. This directory must not
        already exist. (default: ./strelkaAnalysis)
will be glad if someone tells me what arguments are it referring to, and about this error.

[Saikat Banerjee]

ok, so that was some problems with the spaces in the arguments. but now I have a different problem all together, now i get this error

ERROR: Can't resolve directory path for '/media/saikat/Mafia Boy/CSIR IGIB/test_1_lesion_lane1/' from input file argument: '/media/saikat/Mafia Boy/CSIR IGIB/test_1_lesion_lane1/DYP26_L_l1_S1_L001_aln_sorted_Marked_RG_added_recal_applied.bam'

 at ../lib/Utils.pm line 44.
    Utils::errorX("Can't resolve directory path for '/media/saikat/Mafia Boy/CSI"...) called at ./configureStrelkaWorkflow.pl line 145
    main::makeAbsoluteFilePaths(SCALAR(0x1431d48)) called at ./configureStrelkaWorkflow.pl line 151