Different --forceGT vcf on the same cram causing different sites calls besides the forceGT sites.

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Qiaoyan Wang

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Jul 3, 2019, 4:11:39 PM7/3/19
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I was running Strelka by using different --forceGT vcf files on the same cram. No doubt, I got all the forece call sites in the --forceGT files, but I also got different call sites which are in neither of the --forceGT list, Wondering why? Tried run Strelka one more time without --forceGT, result matched one of the previous runs, other than the force calls of course.
Puzzled.
Here I am attaching the PPT for this occurence.

Thanks
Strlka_analysis_slides_20190626.pptx
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