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Edit : Yes, i'm figuring out the torrent thing; currently waiting to hear back from l337 on be able to upload-- a mod has to enable the feature. I love the enthusiasm but we don't need any more separate comment threads asking about a torrent okay ?
edit 3: so l337 denied my request for uploading a torrent. Obviously, there's a healthy interest in a torrent copy. I'll say again, I have the technical understanding of a grandma, so please feel free to advise on what i should do next.
Edit 4: I am uploading a torrent per the advice provided by others below. My internet is not fast, it should take about 36 hours. I would again like to reiterate that I do not know anything about technology or data. Any advice is much appreciated, but please explain things like you would to your grandma. Or a five year old. Better yet, a five year old grandma. thank you)
The files here are bittorrent files. Usea bittorrentclient program to download the contents of the complete ISO imagefiles in a peer-to-peer manner. You should end up with an exact copyof each ISO image as though you'd downloaded it directly via HTTP orFTP.
I share the internet with two other people and I know one of them uses utorrents and is constantly downloading. When her computer is off the internet is substantially faster, would this be because of her torrenting or is it just because there are 3 computers using the wireless.
Maybe your wifi router (I suppose there is one) has an option to set different priorities for different traffic classes e. g. bulk for Torrent, normal for Websites, priority for low-latency online-games. This would be the best solution, because it does not unnecessarily slow down torrent traffic but keeps other traffic at a higher priority. So torrents would be just using the leftover data rate.
They tend to be "greedy" with bandwidth. This is for similar reasons as #1: the torrent has a lot of data to transfer, well beyond what it can send at one time. The torrent client will try to download as much data as it can.
They are bi-directional. In my experience this is the biggest drawback to seeding torrents. Most consumer-level providers give their users asynchronous bandwidth: they allow more traffic to come downstream to the customer than they permit to return upstream. This lets them provide a "faster feeling" experience than a synchronous connection, since most end-user traffic really is downloading. But one key goal of torrents is to serve as much traffic as you pull, which screws up this model. If your upstream bandwidth is being saturated by torrents, your initial requests for connections will take a long time, and everything will feel very, very sluggish.
Most torrent clients are programmed to be very cooperative in mediating these problems. They allow you to regulate things like the number and speed of uploads, downloads, and total connections. Most even have built-in traffic profiling that supply "reasonable" values for these settings, or pre-defined profilers for various speed connections.
Try asking your roommate to configure her client for a slower connection than you have. Try limiting the number of uploads, or the upload bandwidth, and if that doesn't work, just cap the total bandwidth used by the client (say, to 1/3 of your total allocation.)
As others have stated, and I won't necessarily stomp on their answers, your mate's usage will affect your usage - simply because there is only so much bandwidth. They max out the usage, and you're left with a laggy internet.
Look up your Router Manual and how to adjust Quality of Service (QoS) setting. Assuming you have a set Port Number ("Current Port" in the picture above) that doesn't change, you can set the priority of that port to low, thus allowing everything else priority before torrents.
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Hi All,Recently I have been given some targeted iontorrent sequencing data to play with. It's not large amount of data only 18,000 unpaired reads.I have aligned the reads with BWA, pretty much the same as I have always done with illumina fastq files. (about 80% aligned, which seemed a bit low, but whatever, I pushed on).
Not used ion torrent myself but would be curious to see what the fastqc report looked like (quality of data). 80% may be due to poor data and may need some trimming to map more, although BWA-mem trims automatically. Which BWA algorithm have you used?
With Ion torrent and specially with target sequencing, it's normal to have a lot of duplicates. That's why the markduplicate step removes a lot of reads and if you do it, you will miss too much true SNP and INDEL. On the contrary, on Whole Exome Sequencing, you expect to have very few duplicates, and in this case it's appropriate to remove duplicates.For the alignment i suggest you to use bwasw which is specially designed for long reads
Last wednesday, Ion Torrent released a tech note and associated run data with shotgun (single-end) and Mate Pair runs for Escherichia coli K12, substrain MG1655. Both a 3.5 kb and 8.9 kbp insert size, as well as a shotgun library, were sequenced on a 316 chip each. In the tech note, they describe assemblies using different combinations of the data, and show how adding the mate pairs yields assemblies with fewer scaffolds and gaps. The Ion mate-pair protocol is very similar to the one used by 454 Life Sciences for their (unfortunately called) Paired-end libraries: long fragments are circularized using a linker sequence, and sequencing is peformed across this linker, allowing for easy identification of the pair halves.
After extracting the pair halves, I adjusted the files so that newbler would accept them as mate pairs, and performed mapping using newbler (gsMapping/runMapping program). Then I plotted the distances between the mapped mate halves for both libraries:
Next, I set out to perform assemblies. Ion Torrent provided files with reduced amounts of reads so that there would be about 20X and 40X coverage of the 5.4 Mbp genome. I decided to use these files, rather than make my own scaled down versions. I do note, however, that the 20X and 40X files of the mate pair library runs mostly contain mate pair reads, and very few singletons (based on the sff_extract results). Whether Ion determined which reads were mate pair reads by mapping to the reference genome, or by using sff_extract, and only taking reads that were split along the linker, I cannot tell (in a real de novo setting, the second option is the only possible, of course).
With adding the possibility of long insert mate pair library construction and sequencing, Ion Torrent has moved into another niche of Roche/454 Life Sciences. With the fast run time and high throughput of the PGM relative to the 454, Ion Torrent is on its way to become a viable option for de novo small genome sequencing. Apparently, making longer insert libraries (> 10kb) is cumbersome using the Illumina protocols, but not so much with the 454 version, and hopefully also feasible with the Ion Torrent protocol. In addition, the use of a linker allows for easy separation of the mate halves relative to the linker-less Illumina mate-pair reads. It remains to be seen, though, if research groups are able to generate these mate pairs independent of the Ion Torrent labs.
Linux Mint, the desktop user oriented variant of Ubuntu from Ireland, released its new computer operating system version 21 codenamed "Vanessa" late July. This is a list of download links to help you download all editions of it including the alternative mirrors, torrents and checksums so you can run your computer with Linux Mint.
ControllerMate is a controller programming tool that allows users to add custom functionality to standard HID devices (joysticks, trackballs, gamepads, keyboards). Using a highly graphical interface and drag-and-drop editing, users can program controller buttons to perform complex keyboard and mouse sequences. Programming is accomplished using \"building blocks\". Each type of building block performs a different type of function. Building blocks can be individually configured and linked together to perform an endless variety of tasks. Common programming tasks that can be performed with ControllerMate include configuring controller buttons to act like individual keyboard keys, configuring controller buttons to generate simple text, configuring controller axes to act like mouse axes, assigning an AppleScript to a controller button, redefining keyboard keys, assigning a custom acceleration curve to a mouse.","thumbnailUrl":" -3f9c-4f2e-9daa-b80ee47c60ad/imgingest-2915087922826821676.png?auto=webp&fit=crop&height=675&width=1200"},"name":"ControllerMate","applicationCategory":"Drivers","applicationSubCategory":"Game Controllers","image":null,"description":"ControllerMate is a controller programming tool that allows users to add custom functionality to standard HID devices (joysticks, trackballs, gamepads, keyboards). Using a highly graphical interface and drag-and-drop editing, users can program controller buttons to perform complex keyboard and mouse sequences. Programming is accomplished using \"building blocks\". Each type of building block performs a different type of function. Building blocks can be individually configured and linked together to perform an endless variety of tasks. Common programming tasks that can be performed with ControllerMate include configuring controller buttons to act like individual keyboard keys, configuring controller buttons to generate simple text, configuring controller axes to act like mouse axes, assigning an AppleScript to a controller button, redefining keyboard keys, assigning a custom acceleration curve to a mouse.","operatingSystem":"Mac","softwareVersion":"4.11.1","url":" -18490_4-66227.html","author":"@type":"Organization","@id":" -18490_4-66227.html#organization","name":"OrderedBytes","publisher":"@type":"Organization","name":"Download.com","logo":"@type":"ImageObject","height":"250","width":"250","url":" ","thumbnailUrl":" -3f9c-4f2e-9daa-b80ee47c60ad/imgingest-2915087922826821676.png?auto=webp&fit=crop&height=675&width=1200","review":{"@type":"Review","@id":" -18490_4-66227.html#product-review","reviewBody":"ControllerMate for Mac gives you the ability to customize various devices, including the keyboard and mouse, joystick, gamepads, and more, for use with your computer. With a bit of experimenting, you can create all kinds of automated processes to streamline tasks.
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