Campus Stem Cell Lab meeting, Tuesday, April 9

[Sue Gilbert]

Next week, Tuesday, April 9, will be the next talk for this seminar. We will start at noon promptly. We will continue to provide sandwiches and chips starting at 11:40 am (water will be provided but if you want something else to drink please bring your own.) We will be back in The Wisconsin Institutes of Discovery (WID) for these. We will be meeting in the H.F. Deluca Forum in the Town Center on the first floor. Please ask at Information Desk if you are unsure where to go.

 

Date: Tuesday, April 9

 

Speaker: Kaivalya Molugu, SCRMC Trainee, Biomedical Engineering, (Saha lab)

 

Title: Tracking and predicting somatic cell reprogramming using nuclear characteristics

 

Abstract: Reprogramming of human somatic cells to induce pluripotent stem cells (iPSCs) generates valuable cells for disease modeling and regenerative medicine. However, the reprogramming process can be inefficient and noisy, creating many partially reprogrammed intermediates and non-reprogrammed cells in addition to fully reprogrammed iPSCs. Much of the work to identify, evaluate, and enrich for iPSCs during reprogramming relies on methods that fix, destroy, or singularize cell cultures which disrupts each cell’s microenvironment. Here we develop a micropatterned substrate that allows for dynamic live-cell microscopy of thousands of cell subpopulations undergoing reprogramming while preserving many of the biophysical and biochemical cues within the cells’ microenvironment. On this substrate, we were able to both watch and physically confine cells into discrete islands during reprogramming of somatic cells from skin biopsies and blood draws of healthy donors. Using high-content analysis, we identified a combination of eight nuclear characteristics that can be used to track the progression of reprogramming and distinguish partially reprogrammed cells from those that were fully reprogrammed. Non-cell autonomous characteristics such as clustering of nuclei was highly informative in classifying the progression of reprogramming and was used to generate a predictive computational modeling of reprogramming. This approach to track reprogramming in situ using micropatterned substrates could aid in biomanufacturing of therapeutically-relevant iPSCs and be used more generally to understand nuclear and other subcellular changes that accompany human cell fate transitions

 

Reminder: If you are talking this Seminar Series for credit, please check in with Sue Gilbert at the back of the room.

 

Next talk: The next talk of the semester will be on Tuesday, April 16, with our nest speaker, Nisha Iyer, SCRMC Trainee, Biomedical Engineering (Ashton lab), speaking on “Regional Patterning and Engraftment of hPSC-Derived Posterior Central Nervous System Tissue." This talk will be in the Wisconsin Institutes of Discovery (WID), H.F. Deluca Forum in the Town Center on the first floor, and will include sandwiches.

 

A list of the talks for the semester is found at:

https://stemcells.wisc.edu/campus-lab-meeting-schedule/

Campus Lab Meeting Schedule – SCRMC – UW–Madison stemcells.wisc.edu This event is held in the center of campus at the new Wisconsin Institutes for Discovery (most weeks), making it easy for all groups to attend. It currently attracts up to 100 students, post-doctoral fellows, staff and faculty each week. The structure is flexible and will encompass presentations of current research as well as discussion…

 

Thank you for supporting this venue for the sharing of information.

 

Sue Gilbert
Office Operations Associate
Stem Cell and Regenerative Medicine Center
8457 WIMR II
1111 Highland Avenue       
Madison, WI 53705-2275

Phone: 608.263.2982

"Somewhere, something incredible is waiting to be known."

Carl Sagan