I am seeing something that is making me wonder. The plot that I got using QIIME 2 is very different from fatsqc. The only difference in the data is that I demutiplexed with qiime first because that was how qiime gave me the report but in fastqc, I used the multiplexed data all together. What I get using fastqc is similar to the plots out sequencing center gave us.
After removing the older version of fastqc using apt purge fastqc, I downloaded the latest version folder and extracted the files in my Users folder. To make it recognizable by Ubuntu, I set the FastQC directory in my PATH using
This function will transfer the default template to the provided directory andproduce a single .html file containing interactive summary plots of anyFastQC output found in the directory.FastQC output can be *fastqc.zip files or the same files extracted asindividual directories.
This pipeline runs fastqc in parallel on the input fastq files (paired or not)and then execute multiqc. A brief sequana summary report is also produced.sYou may use falco instead of fastqc. This is experimental but seem to work forIllumina/FastQ files.
Complete the rule below so that the output filenames are correctly produced. You will need to addextra commands to the shell part after running fastqc. Do not alter the output or inputparts of the rule.
FastQC can be invoked from the command line by typing fastqc , and it will produce an html report and associated zip file containing data, plots, and some ancillary files. The zip file contains an Images folder where the plots that become incorporated into the html report are stored. They are:
The zipped folder also contains a file called fastqc_data.txt and summary.txt. fastqc_data.txt contains the raw data and statistics while summary.txt summarizes which tests have been passed.
Hi, I am having trouble running FastQC on my data. I thought that it was already installed on my instance, but when I tried to run it I got a message telling me to install it using
sudo apt install fastqc
which I did, and it seemed to be successful. Now when I run fastQC, I get the following message
To view the Fastqc results, you may open the fastqc_report.html file ina web browser or the summary.txt file (located in the zipped outputarchive) on the command line. For assistance copying files to your localmachine, please see the Moving Data page.