Query related to Lorikeet viewer PTMprophet results in TPP

[Mehar Un Nissa]

Hello Sir/Madam,

We used TPP and PTMprophet for PTM analysis.

PTM  taken (Phosphorylation- STY).

I want to see the spectra in Lorikeet viewer but it is showing following error.

Please help me solve this. 

Looking forward to hearing from you. 

Thanks and regards,

Mehar Un Nissa

Research Scholar,

Proteomics Lab.

Department of Biosciences and Bioengineering,

Indian Institute of Technology, Bombay-400076

[Mehar Un Nissa]

Dear Sir/Madam,

Please reply to my query in the above mail. 

Thanks and regards,

Mehar Un Nissa

Research Scholar,

Proteomics Lab.

Department of Biosciences and Bioengineering,

Indian Institute of Technology, Bombay-400076

[Luis Mendoza]

Hello,

It seems that Lorikeet is unable to find the mzML file to extract and display the spectrum.  Did you move the files after you conducted the analysis?

If you look at the bottom of your screenshot, you can see that the file must be located under "/proteomics/jpanga/....."  Can you confirm that the file is in that location on your file server?

Cheers,

--Luis

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[David Shteynberg]

It appears that you are trying to view the results on a Windows machine but the data was processed on Linux with absolute paths to the mzML data files.  Unless the files are properly referenced at their correct locations on your computer, this will not work.   You might consider running the TPP Tools -> Utilities -> Update Paths application on the system to which the data has been copied.  Let us know if that solves the problem.

-David

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