Regarding total intensity normalization in LIBRA

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Debojyoti Pal

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Jul 31, 2023, 2:09:47 PM7/31/23

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Dear all

I am quantifying an iTRAQ run, where one of the channel seems to be having lower intensity across the board (around 20%). I am thinking of the TIC normalization (total intensity), but it is mentioned as "not recommended" in LIBRA settings.

Why is it so? If I am mixing equal labelled peptide, what is wrong in TIC, assuming one of the peptides labeled poorly? What is the alternative here if that is not suitable?

Also, Can I see the total intensity of each channel somewhere?

Regards

Debojyoti

Debojyoti Pal

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Aug 1, 2023, 12:33:19 AM8/1/23

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Can anyone help me with this, please?

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