could not open database file "/dbase/IPI/ipi.HUMAN.fasta.v2.31" (No such file or directory)

[Zifu Zhao]

Hello everyone,

I am learning to use TPP 7.1 by analyzing the example data from the article "TPP: Example Data Analysis." Converting the original search results to pepXML was successful, but there was an error when validating peptide identifications using PeptideProphet. It could not open the database file "/dbase/IPI/ipi.HUMAN.fasta.v2.31" (No such file or directory).

I followed the installation instructions, so it's unlikely that there are any issues. However, my computer does not have IIS services, which means that the path C:\Inetpub\wwwroot\ISB\data does not exist. I'm not sure if this is related to the problem.

If anyone knows how to solve this issue, please contact me.

Thank you.

Regards,
Zifu Zhao
Graduate Student
School of Life Sciences, Wuhan University
China
Hubei
Wuhan