SpectrasT can't recognize Protein N-term acetylation modification

[RayS]

I'm using SpectraST in TPP for building a splib format file from a bibliospec MS2 format file. While converting  it issues error message for peptides with Protein N-term modifications

"MS2 IMPORT: Peptide ID has unknown modification: "M[+42.01056]FLVNSFLK". Skipped spectrum"

I guess I can write this as user defined modification in a file and specify the file name with -M option in the command line

M[n]|+42.01056|Protein_N-term

and the following command 

spectrast.exe -cNmyLib -M SpectraST_MyMod.txt myLib.ms2

I guess the syntax I wrote is wrong because SpectraST still skips those modifications.

Can anyone help me with the correct synatx and command please?

Thanks

[Eric Deutsch]

Hi Ray, I suspect that SpectraST will recognize this notation:

 

n[42]MFLVNSFLK

 

N terminal modifications are shown after an n. Side-chain modifications are shown after the residue.

 

Eric

--
You received this message because you are subscribed to the Google Groups "spctools-discuss" group.
To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discu...@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/c7153b98-145d-435f-bcb4-75b5dca2a893%40googlegroups.com.