Precursor Intensity after Comet search

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Will Comstock

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Sep 16, 2021, 11:11:56 AMSep 16
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Hello,

After Comet searching mzXML files, appending the "Precursor Intensity" column in the pepXML viewer reveals that it contains no values. Is there a way to make Comet retrieve/retain precursor intensities for later use? Would running XPRESS in label-free mode essentially accomplish this? I am using TPP 6.0 for these analyses. 

Respectfully,
-Will Comstock

David Shteynberg

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Sep 16, 2021, 11:31:43 AMSep 16
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Hi Will,

You can use option -PREC in advanced options for xinteract "Analyze Peptides" results to populate this column.    Alternatively you can run InteractParser with -I flag to get the same values in the pepXML results.

Cheers,
-David

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Will Comstock

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Sep 16, 2021, 11:47:51 AMSep 16
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Thank you David, exactly what I was looking for!

Best,
-Will

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