Runtime to terminate :-(

[Alex]

I think my post didn't show up correctly. Here it goes again!

Hi TTP community,

I'm running some ASAPratio quant on a 2D-LC-MSMS experiment on windows XP running TPP v4.5 RAPTURE rev 2, Build 201202031108 (MinGW). After a

 couple of hours analysis I get the following error. Has somebody run into this problem before??? Any ideas much appreciated!!!

Alex

Error Message:

This application has requested the Runtime to terminate it in an unusual way.

Please contact the application's support team for more information.

command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F -C -r0.1 -S -mK160.15137n33.06425" failed: No such process

command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F -C -r0.1 -S -mK160.15137n33.06425" exited with non-zero exit code: 3

QUIT - the job is incomplete

command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -Op -I1 -I4 -I5 -A-lKn-F-C-r0.1-S-mK160.15137n33.06425 2DYeast01.tandem.pep.xml

2DYeast02.tandem.pep.xml 2DYeast03.tandem.pep.xml 2DYeast04.tandem.pep.xml 2DYeast05.tandem.pep.xml 2DYeast06.tandem.pep.xml 

2DYeast07.tandem.pep.xml 2DYeast08.tandem.pep.xml 2DYeast09.tandem.pep.xml 2DYeast10.tandem.pep.xml 2DYeast11.tandem.pep.xml 

2DYeast12.tandem.pep.xml 2DYeast13.tandem.pep.xml 2DYeast14.tandem.pep.xml 2DYeast15.tandem.pep.xml 2DYeast16.tandem.pep.xml 

2DYeast17.tandem.pep.xml 2DYeast18.tandem.pep.xml 2DYeast19.tandem.pep.xml 2DYeast20.tandem.pep.xml 2DYeast21.tandem.pep.xml 

2DYeast22.tandem.pep.xml 2DYeast23.tandem.pep.xml H2DYeast01.tandem.pep.xml H2DYeast02.tandem.pep.xml H2DYeast03.tandem.pep.xml 

H2DYeast04.tandem.pep.xml H2DYeast05.tandem.pep.xml H2DYeast06.tandem.pep.xml H2DYeast07.tandem.pep.xml H2DYeast08.tandem.pep.xml 

H2DYeast09.tandem.pep.xml H2DYeast10.tandem.pep.xml H2DYeast11.tandem.pep.xml H2DYeast12.tandem.pep.xml H2DYeast13.tandem.pep.xml 

H2DYeast14.tandem.pep.xml H2DYeast15.tandem.pep.xml H2DYeast16.tandem.pep.xml H2DYeast17.tandem.pep.xml H2DYeast18.tandem.pep.xml 

H2DYeast19.tandem.pep.xml H2DYeast20.tandem.pep.xml H2DYeast21.tandem.pep.xml H2DYeast22.tandem.pep.xml H2DYeast23.tandem.pep.xml" failed: No 

such process

[Brian Hampton]

I've never gotten the static option to work.  I'm not sure how the static option is intended to be used..... correctly e.g. What type of data set.

If you are doing two searches one with the static light and the second with the static heavy isotope labels, you will have to redo the search and do it as per the Tandem API where you set the light to be a static mod and the mass difference between the heavy and light to be a variable mod.  In addition to giving results ASAPRatio can quantitate, it has been noted that the second search method is more statistically sound because it increases the size of the database as a result of the variable mods.

Using the second search strategy has reduced ASAPRatio processing time on my data sets from  many hours to about a half hour.  Also, using the static option on Linux w/ TPP 4.5.2 ASAPRatio would complete after hours.  But I have problems with the viewers not working in Linux so after switching to Win for the quan analysis, using TPP 4.6.1 the static option always kicks out an error and quits.  Feeding it search results as mentioned above it all works and completes much more quickly.

Why the static option doesn't work on our data sets is a mystery to me.

Brian

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[Alex]

Thanks Brain for your quick reply,

I'm running a dimethylation experiment (Ligth+28, Heavy+32). Until now, I have been acquiring data with a QTRAP2000, and everything worked nicely using the static approach (but not much proteome coverage with this MS). We now got a VelosPRO Orbitrap, and I am reacquiring the same samples!!!. I'm running X!Tandem, and DB search worked so far for protein ID. When I ANALYZE peptides with ASAPratio, things crash after 2-3 hours with this message. Until now, I have always been using the STATIC approach (according to the many threads I have reviewed on this topic), where I repeat the DB search for the light and heavy using two different parameters search. It used to work fine with the QTRAP2000 data. Of course, my files with the orbitrap are much larger, so I was suspecting I was running into some memory issue (Im not sure what this Runtime is all about).

I'll try with your approach, if you have any other ideas, welcome!!!

Alex

[kshitiz tyagi]

just curiois! have u tried XPRESS?? On large datasets, in our lab, ASAP gave us a lot of problems while XPRESS worked. 

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Kshitiz Tyagi

[Alex]

no, have not tried Xpress. I preferred the way to visualize and analyze data on ASAP, and preferred it.... while it worked! thanks for the comment anyway

[David Shteynberg]

Hi Alex,

Have you tried that latest version of TPP?  If that one still fails then your processing could be exposing a potential new bug that we would certainly like to fix.  Can you check that all your data files are there, non-empty, readable and complete.  If there is a corrupted mzML/mzXML file and ASAPRatio cannot read a certain spectrum from the file this could also cause a bad crash.  An easy way to troubleshoot this is to sort by file type and compare the sizes of the mzML/mzXML files, are they all about the same or are some much smaller.  If it worked on this type of analysis before it should work again regardless of the instrument you used to acquire your data.

Good luck,

-David

[Alex]

David,

Thanks for the reply. I downloaded the latest version of TPP and rerun. I checked on all the data and they seem to be completer, all have similar size (no blank files!). I repeated ASAP many times, and it seems to be haulting at different times, this time at static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast16.mzML (see error log below), but others at 18mzML, so the error is somewhat deceiving. Memory issue maybe?

Any help to unravel this mystery will be much appreciated!!!, Thanks!

Alex

c:\Inetpub\tpp-bin\xinteract (TPP v4.6 OCCUPY rev 1, Build 201209261035 (MinGW))

running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" "2DYeast01.tandem.pep.xml" "2DYeast02.tandem.pep.xml" "2DYeast03.tandem.pep.xml" "2DYeast04.tandem.pep.xml" "2DYeast05.tandem.pep.xml" "2DYeast06.tandem.pep.xml" "2DYeast07.tandem.pep.xml" "2DYeast08.tandem.pep.xml" "2DYeast09.tandem.pep.xml" "2DYeast10.tandem.pep.xml" "2DYeast11.tandem.pep.xml" "2DYeast12.tandem.pep.xml" "2DYeast13.tandem.pep.xml" "2DYeast14.tandem.pep.xml" "2DYeast15.tandem.pep.xml" "2DYeast16.tandem.pep.xml" "2DYeast17.tandem.pep.xml" "2DYeast18.tandem.pep.xml" "H2DYeast01.tandem.pep.xml" "H2DYeast02.tandem.pep.xml" "H2DYeast03.tandem.pep.xml" "H2DYeast04.tandem.pep.xml" "H2DYeast05.tandem.pep.xml" "H2DYeast06.tandem.pep.xml" "H2DYeast07.tandem.pep.xml" "H2DYeast08.tandem.pep.xml" "H2DYeast09.tandem.pep.xml" "H2DYeast10.tandem.pep.xml" "H2DYeast11.tandem.pep.xml" "H2DYeast12.tandem.pep.xml" "H2DYeast13.tandem.pep.xml" "H2DYeast14.tandem.pep.xml" "H2DYeast15.tandem.pep.xml" "H2DYeast16.tandem.pep.xml" "H2DYeast17.tandem.pep.xml" "H2DYeast18.tandem.pep.xml" -L"7""

 file 1: 2DYeast01.tandem.pep.xml

 file 2: 2DYeast02.tandem.pep.xml

 file 3: 2DYeast03.tandem.pep.xml

 file 4: 2DYeast04.tandem.pep.xml

 file 5: 2DYeast05.tandem.pep.xml

 file 6: 2DYeast06.tandem.pep.xml

 file 7: 2DYeast07.tandem.pep.xml

 file 8: 2DYeast08.tandem.pep.xml

 file 9: 2DYeast09.tandem.pep.xml

 file 10: 2DYeast10.tandem.pep.xml

 file 11: 2DYeast11.tandem.pep.xml

 file 12: 2DYeast12.tandem.pep.xml

 file 13: 2DYeast13.tandem.pep.xml

 file 14: 2DYeast14.tandem.pep.xml

 file 15: 2DYeast15.tandem.pep.xml

 file 16: 2DYeast16.tandem.pep.xml

 file 17: 2DYeast17.tandem.pep.xml

 file 18: 2DYeast18.tandem.pep.xml

 file 19: H2DYeast01.tandem.pep.xml

 file 20: H2DYeast02.tandem.pep.xml

 file 21: H2DYeast03.tandem.pep.xml

 file 22: H2DYeast04.tandem.pep.xml

 file 23: H2DYeast05.tandem.pep.xml

 file 24: H2DYeast06.tandem.pep.xml

 file 25: H2DYeast07.tandem.pep.xml

 file 26: H2DYeast08.tandem.pep.xml

 file 27: H2DYeast09.tandem.pep.xml

 file 28: H2DYeast10.tandem.pep.xml

 file 29: H2DYeast11.tandem.pep.xml

 file 30: H2DYeast12.tandem.pep.xml

 file 31: H2DYeast13.tandem.pep.xml

 file 32: H2DYeast14.tandem.pep.xml

 file 33: H2DYeast15.tandem.pep.xml

 file 34: H2DYeast16.tandem.pep.xml

 file 35: H2DYeast17.tandem.pep.xml

 file 36: H2DYeast18.tandem.pep.xml

 processed altogether 313436 results

 results written to file c:/Inetpub/wwwroot/ISB/data/121119YEAST/interact.pep.xml

 direct your browser to http://localhost/ISB/data/121119YEAST/interact.pep.shtml

command completed in 114 sec 

running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml""

command completed in 2 sec 

running: "C:/Inetpub/tpp-bin/RefreshParser "interact.pep.xml" "c:/Inetpub/wwwroot/ISB/data/dbase/Yeast/YEAST.fasta""

  - Searching the tree...

  - Linking duplicate entries...  - Printing results...

  - Building Commentz-Walter keyword tree...command completed in 64 sec 

running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" MINPROB=0.05 ACCMASS IGNORECHG=1 IGNORECHG=4 IGNORECHG=5"

using Accurate Mass Bins

Ignoring charge 1+ spectra.

Ignoring charge 4+ spectra.

Ignoring charge 5+ spectra.

 (X! Tandem (k-score))

adding Accurate Mass mixture distr

init with X! Tandem (k-score) trypsin 

MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN, Ionization: UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN

 PeptideProphet  (TPP v4.6 OCCUPY rev 1, Build 201209261035 (MinGW)) AKeller@ISB

 read in 0 1+, 196963 2+, 109817 3+, 0 4+, 0 5+, 0 6+, and 0 7+ spectra.

Initialising statistical models ...

negmean = 0.0533258

negmean = -3.13661

negmean = -3.85342

negmean = -0.2

negmean = -0.2

negmean = -0.2

negmean = -0.2

Iterations: .........10.........20.........30

model complete after 31 iterations

command completed in 292 sec 

running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i interact.pep.xml"

Analyzing interact.pep.xml ...

Reading Accurate Mass Model model +2 ...

Reading Accurate Mass Model model +3 ...

Reading Accurate Mass Model model +6 ...

Reading Accurate Mass Model model +7 ...

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast01 (X! Tandem (k-score))"...

  => Total of 152 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast02 (X! Tandem (k-score))"...

  => Total of 260 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast03 (X! Tandem (k-score))"...

  => Total of 627 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast04 (X! Tandem (k-score))"...

  => Total of 890 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast05 (X! Tandem (k-score))"...

  => Total of 1158 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast06 (X! Tandem (k-score))"...

  => Total of 2329 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast07 (X! Tandem (k-score))"...

  => Total of 5279 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast08 (X! Tandem (k-score))"...

  => Total of 7834 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast09 (X! Tandem (k-score))"...

  => Total of 9551 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast10 (X! Tandem (k-score))"...

  => Total of 10974 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast11 (X! Tandem (k-score))"...

  => Total of 12378 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast12 (X! Tandem (k-score))"...

  => Total of 13705 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast13 (X! Tandem (k-score))"...

  => Total of 14791 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast14 (X! Tandem (k-score))"...

  => Total of 15572 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast15 (X! Tandem (k-score))"...

  => Total of 16177 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast16 (X! Tandem (k-score))"...

  => Total of 16690 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast17 (X! Tandem (k-score))"...

  => Total of 17270 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast18 (X! Tandem (k-score))"...

  => Total of 17762 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast01 (X! Tandem (k-score))"...

  => Total of 17899 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast02 (X! Tandem (k-score))"...

  => Total of 18026 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast03 (X! Tandem (k-score))"...

  => Total of 18284 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast04 (X! Tandem (k-score))"...

  => Total of 18524 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast05 (X! Tandem (k-score))"...

  => Total of 18783 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast06 (X! Tandem (k-score))"...

  => Total of 19862 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast07 (X! Tandem (k-score))"...

  => Total of 22746 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast08 (X! Tandem (k-score))"...

  => Total of 25483 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast09 (X! Tandem (k-score))"...

  => Total of 27219 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast10 (X! Tandem (k-score))"...

  => Total of 28602 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast11 (X! Tandem (k-score))"...

  => Total of 29919 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast12 (X! Tandem (k-score))"...

  => Total of 31155 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast13 (X! Tandem (k-score))"...

  => Total of 32230 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast14 (X! Tandem (k-score))"...

  => Total of 33060 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast15 (X! Tandem (k-score))"...

  => Total of 33658 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast16 (X! Tandem (k-score))"...

  => Total of 34102 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast17 (X! Tandem (k-score))"...

  => Total of 34688 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

Parsing search results "c:/Inetpub/wwwroot/ISB/data/121119YEAST/H2DYeast18 (X! Tandem (k-score))"...

  => Total of 35147 hits.

  => Total of 0 decoy hits.

  => Total of 0 excluded hits.

command completed in 11 sec 

running: "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F -C -r0.2 -S -mK160.15137n33.06425"

K 156.126 160.151

n 29.0391 33.0643

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast01.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast02.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast03.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast04.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast05.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast06.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast07.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast08.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast09.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast10.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast11.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast12.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast13.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast14.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast15.mzML

static light label for file c:/Inetpub/wwwroot/ISB/data/121119YEAST/2DYeast16.mzML

This application has requested the Runtime to terminate it in an unusual way.

Please contact the application's support team for more information.

command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F -C -r0.2 -S -mK160.15137n33.06425" failed: No such process

command "C:/Inetpub/tpp-bin/ASAPRatioPeptideParser "interact.pep.xml" -lKn -F -C -r0.2 -S -mK160.15137n33.06425" exited with non-zero exit code: 3

QUIT - the job is incomplete

command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -OAp -I1 -I4 -I5 -A-lKn-F-C-r0.2-S-mK160.15137n33.06425 2DYeast01.tandem.pep.xml 2DYeast02.tandem.pep.xml 2DYeast03.tandem.pep.xml 2DYeast04.tandem.pep.xml 2DYeast05.tandem.pep.xml 2DYeast06.tandem.pep.xml 2DYeast07.tandem.pep.xml 2DYeast08.tandem.pep.xml 2DYeast09.tandem.pep.xml 2DYeast10.tandem.pep.xml 2DYeast11.tandem.pep.xml 2DYeast12.tandem.pep.xml 2DYeast13.tandem.pep.xml 2DYeast14.tandem.pep.xml 2DYeast15.tandem.pep.xml 2DYeast16.tandem.pep.xml 2DYeast17.tandem.pep.xml 2DYeast18.tandem.pep.xml H2DYeast01.tandem.pep.xml H2DYeast02.tandem.pep.xml H2DYeast03.tandem.pep.xml H2DYeast04.tandem.pep.xml H2DYeast05.tandem.pep.xml H2DYeast06.tandem.pep.xml H2DYeast07.tandem.pep.xml H2DYeast08.tandem.pep.xml H2DYeast09.tandem.pep.xml H2DYeast10.tandem.pep.xml H2DYeast11.tandem.pep.xml H2DYeast12.tandem.pep.xml H2DYeast13.tandem.pep.xml H2DYeast14.tandem.pep.xml H2DYeast15.tandem.pep.xml H2DYeast16.tandem.pep.xml H2DYeast17.tandem.pep.xml H2DYeast18.tandem.pep.xml" failed: No such process

[David Shteynberg]

Hi Alex,

Please try rerunning once without the static option to see if it still fails.   The static flag is on its way out and could well be the problem.

Thanks,

David

To view this discussion on the web visit https://groups.google.com/d/msg/spctools-discuss/-/IkcaFAFiSF8J.