Related to St Peter tool in TPP

[Mehar Un Nissa]

Hello TPP Team,

I want to use St. Peter tool in TPP for Label free quantification of some dataset. 

Once I go to TPP Tools--> Quantify Label Free (MS2), the web page redirected to the login page when I login again some error is coming as under:

Could you please through some light in this regard. 

Looking forward to hearing from you. 

Thanks and regards,

Mehar Un Nissa

Research Scholar,

Proteomics Lab.

Department of Biosciences and Bioengineering,

Indian Institute of Technology, Bombay-400076

[Luis Mendoza]

Hello Mehar,

It seems that there was an issue with reading your user session cookie -- perhaps you followed an old link, reinstalled TPP while logged in, or your browser does not support cookies.

I suggest starting a new browser session and try to run TPP again; let us know if the issue persists.  Has this happened more than once?

Cheers,

--Luis

--
You received this message because you are subscribed to the Google Groups "spctools-discuss" group.
To unsubscribe from this group and stop receiving emails from it, send an email to spctools-discu...@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CAH%2BQocoJzhCopwYEwOYDH80v164JjdmEoE2brYEH32tv2OzpoQ%40mail.gmail.com.

[Mehar Un Nissa]

Thanks Luis.

I started TPP in a new browser and it worked. 

Further for my dataset, I need protein abundance to check the comparative protein expression in each sample. 

While using St Peter tool, on what basis the option "Amount of protein sample measured" is decided.

I saw in one of the tutorial that they have taken it as 2.0 micrograms. 

For initial MS run, I have injected 1 microgram peptide for obtaining shotgun data, so what value I should put here for quantification of proteins present in my sample. 

(Queried option is highlighted in this snapshot)

Looking forward to hearing from you. 

Thanks and regards,

Mehar Un Nissa

Research Scholar,

Proteomics Lab.

Department of Biosciences and Bioengineering,

Indian Institute of Technology, Bombay-400076

To view this discussion on the web visit https://groups.google.com/d/msgid/spctools-discuss/CACyS9bqKP9g586_APODu%2BsGmdV5DxxELos-_705JbX9s6%3DgLiw%40mail.gmail.com.

[Jason Winget]

You would put 1 in that box, since you know you injected 1 ug.