how to convert profile data in RAW to centroid data in mzXML?

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zeyu sun

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Mar 3, 2015, 5:34:14 AM3/3/15
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Hi, 
I'm wondering if there is any tools can convert profile data in RAW to centroid data in mzXML? I have tried ProteoWizard, but it didn't change profile data to centroid data. I current don't have access to Xcailbur. 

thanks!

SunSun

Frédéric DELOLME

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Mar 3, 2015, 6:11:46 AM3/3/15
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Hi SunSun,

Did you try to check the "Centroid all scans (MS1 and MS2) -- meaningful only if data was acquired in profile mode" in the TPP Conversions options ?

Fred
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Eric Deutsch

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Mar 3, 2015, 10:56:00 AM3/3/15
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ProteoWizard’s msconvert *can* convert profile to centroided. That it didn’t for you means that either incorrect settings were applied or there is some other glitch to be fixed.

zeyu sun

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Mar 3, 2015, 7:49:51 PM3/3/15
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Thank you Eric, I just found out the Peak picking filter in msconvert will centroid the data. I guess the problem is solved!

thank you all!

SunSun

Robert Willows

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Mar 9, 2015, 12:36:39 PM3/9/15
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Thanks Miguel,

I've added these in an have confirmed some joins but not others.

Robert
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