Re: Problems with N_A quantification on ASAPRatio

[julia...@yahoo.com.br]

I also use AsapRatio and I frequently have NAs in my results and as you sad I am wondering of it is an experimental problem. Does anyone have experienced this problem before? What should I do to avoid it?

 

regards,

 

Julia

 

Em quinta-feira, 26 de julho de 2012 14h58min44s UTC-3, Daniel FS escreveu:

I'm using TPP v4.4 vuvuzela rev 1 and I'm working with SILAC cells. Everything is going right until the quantification time, when my result seems to be very bad. After 8 runs with different proportions of heavy/light cells (1:1, 2:1) in a following conditions (Thermo Orbitrap -> Mascot Search with Fixed Mod. on Carbamidomethyl and Variable Mod. on Label(6)K and Oxidation(M)  -> Analysis Pipeline with XPRESS mass diference (K) 6.02 and ASAPRatio with change label K and Specific mass residue K 134.114), my results are around 70% N_A, and just 20% really quantified. My question is: why there is so much N_A in my results? I know that N_A is something about cannot quantify, but what it is exactly? Is it something experimental or is it a bad use of TPP?

Thanks in advance,

Daniel