Hello!
I've been trying to run SPADA but I keep getting this error. What exactly does this mean and how should I fix it? Thanks!
===== setting up environment variables ===== using Mtruncatula matrix will run Augustus_evidence: Augustus will run GeneWise_SplicePredictor: GeneWise;SplicePredictor [07:31:43] ########## Starting pipeline ########## [07:31:43] ##### Stage 1 [Pre-processing] ##### [07:31:43] creating symlink to: 01_refseq.fas [07:31:43] translating sequences in 6 reading frames [07:31:44] extracting ORFs from translated genomic sequence [07:31:46] creating symlink to: 51_gene.gff
gff2gtb.pl -i 51_gene.gff -o 61_gene.gtb Use of uninitialized value $s in substr at /usr/local/share/perl/5.26.1/Data/Table.pm line 1660.
gtb2gff.pl -i 61_gene.gtb -o 62_gene.gff 0 genes : 0 models [07:31:47] extracting ORFs from predicted protein sequence [07:31:47] ##### Stage 2 [Motif Mining] ##### [07:31:47] running hmmsearch against 12_orf_genome.fas [07:31:47] parsing HMM output [07:31:47] calculating alignment coordinates [07:31:47] calculating alignment coordinates [07:31:47] transforming coordinates (Amino Acid -> Nucleotide) [07:31:47] recovering to global coordinate [07:31:47] 105 in total [07:31:47] refining HMM hits [07:31:47] preparing for hit tiling [07:31:47] tiling HMM hits [07:31:47] removing noise hits [07:31:47] 10 removed / 95 passed [07:31:47] removing hits on wrong reading frames [07:31:47] 0 removed / 95 passed [07:31:47] re-formatting hit info malformat location string: protein
Kind regards,
Diya