error running Spada

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Clarissa B.

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Aug 3, 2016, 12:33:40 PM8/3/16
to SPADA

Dear all, I installed all the tools and perl modules.
When I tried the command:

perl spada.pl --cfg conf.txt --org test --fas ./test/01_refseq.fa --gff ./test/51_gene.gff

I got the error message:

Possible precedence issue with control flow operator at /usr/local/share/perl/5.22.1/Bio/DB/IndexedBase.pm line 791.
Usage:
      spada.pl [-help] [options...]

      Options:
        -h (--help)    brief help message
        -c (--cfg)     config file
                         defaut: 'cfg.txt'
        -d (--dir)     SPADA output directory
                         defaut: 'spada.crp.test'
        -p (--hmm)     directory with profile alignments and HMM files
                         defaut: 'hmm.crp'
        -f (--fas)     genome sequence (FASTA) file
                         defaut: 'test/01_refseq.fas'
        -g (--gff)     gene annotation file (GFF3 format, not required)
                         defaut: ''
        -o (--org)     organism: this tells GeneMark / GlimmerHMM / GeneID
                         which *.mod / training dir / *.param file to use
                         supported: 'Athaliana', 'Mtruncatula', 'Osativa'
                         defaut: 'Athaliana'
        -s (--sp)      if set, predictions without signal peptide are filtered
                         default: TRUE
        -e (--evalue)  E-value threshold
                         default: 0.05
        -t (--threads) threads (precessors) to use
                         default: 1
        -m (--method)  gene prediction programs to run (semicolon-seperated)
                       supported:
                         'Augustus_evidence', 'GeneWise_SplicePredictor',
                         'Augustus_de_novo', 'GeneMark', 'GlimmerHMM', 'GeneID'
                       default: 'Augustus_evidence;GeneWise_SplicePredictor'


Any ideas to solve it?

Thanks
Clarissa
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