Dear all, I installed all the tools and perl modules.
When I tried the command:
perl
spada.pl --cfg conf.txt --org test --fas ./test/01_refseq.fa --gff ./test/51_gene.gff
I got the error message:
Possible precedence issue with control flow operator at /usr/local/share/perl/5.22.1/Bio/DB/IndexedBase.pm line 791.
Usage:
spada.pl [-help] [options...]
Options:
-h (--help) brief help message
-c (--cfg) config file
defaut: 'cfg.txt'
-d (--dir) SPADA output directory
defaut: 'spada.crp.test'
-p (--hmm) directory with profile alignments and HMM files
defaut: 'hmm.crp'
-f (--fas) genome sequence (FASTA) file
defaut: 'test/01_refseq.fas'
-g (--gff) gene annotation file (GFF3 format, not required)
defaut: ''
-o (--org) organism: this tells GeneMark / GlimmerHMM / GeneID
which *.mod / training dir / *.param file to use
supported: 'Athaliana', 'Mtruncatula', 'Osativa'
defaut: 'Athaliana'
-s (--sp) if set, predictions without signal peptide are filtered
default: TRUE
-e (--evalue) E-value threshold
default: 0.05
-t (--threads) threads (precessors) to use
default: 1
-m (--method) gene prediction programs to run (semicolon-seperated)
supported:
'Augustus_evidence', 'GeneWise_SplicePredictor',
'Augustus_de_novo', 'GeneMark', 'GlimmerHMM', 'GeneID'
default: 'Augustus_evidence;GeneWise_SplicePredictor'
Any ideas to solve it?
Thanks
Clarissa