FFPE slides preparation

[Erin Wu]

Hi,

I have some questions about your method details of slide preparation. Have you baken slides at some given temprature to dry and mount sections on slides. How long and what temprature? I wonder if I bake the slides in an oven before staining because temprature will cause further RNA degradation? You describe "Slides were stored overnight in a nitrogen chamber" in your article, I want to know the role of this step. Thanks!

[Chunfang zhu]

Hi Erin,

We never bake the slides. We air dry the slides for 1-3hours after mounting sections on slides.

Our slides were stored overnight in a nitrogen chamber in order to make the moisture-free atmosphere and keep the slides dry. 

Best,

chunfang

On Sun, Oct 18, 2020 at 9:27 PM Erin Wu <zhongq...@gmail.com> wrote:

Hi,

I have some questions about your method details of slide preparation. Have you baken slides at some given temprature to dry and mount sections on slides. How long and what temprature? I wonder if I bake the slides in an oven before staining because temprature will cause further RNA degradation? You describe "Slides were stored overnight in a nitrogen chamber" in your article, I want to know the role of this step. Thanks!

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[Erin Wu]

OK! Sincerely thanks for your reply, can I process staining immediately after air dry 1-3h? nitrogen chamber overnight can make sections tightly attach to slides, is that right?

[Chunfang zhu]

You can stain the slides immediately after completely dry.  The tissue can fall off from slides sometimes during staining if it doesn't dry completely.

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